cycle of the parasite, or that it may be propagated through successive generations of mosquitoes.
It is well known that persons engaged in digging canals, railroad cuts, etc., in malarious regions are especially liable to be attacked with one or the other of the forms of malarial fever. This may be due to the fact that the digging operations result in the formation of little pools suitable for the development of the eggs of Anopheles, but another explanation has been offered. Ross and others have found in infected mosquitoes certain bodies, described by Ross as 'black spores/ which resist decomposition and which may be resting spores capable of retaining their vitality for a long time. The suggestion is that these 'black spores' or other encysted reproductive bodies may have been deposited in the soil by mosquitoes long since defunct 'and that in moving the soil these dormant parasites are set at liberty and so in air, in water or otherwise, gain access to the workmen engaged' (Manson). This hypothesis is not supported by recent observations, which indicate that infection in man occurs only as a result of inoculation through the bite of an infected mosquito. The question is whether malarial fevers can be contracted in marshy localities independently of the mosquito, which has been demonstrated to be an intermediate host of the malarial parasite? Is this parasite present in the air or water in such localities as well as in the bodies of infected mosquitoes? Its presence has never been demonstrated by the microscope; but this fact has little value in view of the great variety of micro-organisms present in marsh water or suspended in the air everywhere near the surface of the ground, and the difficulty of recognizing the elementary reproductive bodies by which the various species are maintained through successive generations. It would appear that a crucial experiment for the determination of this question would be to expose healthy individuals in a malarious region and to exclude the mosquito by some appropriate means. This experiment has been made during the past summer and the result, up to the present time, has been reported by Manson in the London 'Lancet' of September 29. Five healthy individuals have lived in a hut on the Roman Campagna since early in the month of July. They have been protected against mosquito bites by mosquito-netting screens in the doors and windows and by mosquito bars over the beds. They go about freely during the daytime, but remain in their protected hut from sunset to sunrise. At the time Manson made his report all these individuals remained in perfect health. It has long been known that laborers could come from the villages in the mountainous regions near the Roman Campagna and work during the day, returning to their homes at night, without great danger of contracting the fever, while those who remained on the Campagna at night ran great risk of falling sick with fever, as a result of 'exposure to the night air.' What has already been said makes it appear extremely probable
