File:Analysis-of-Human-Cytomegalovirus-Encoded-SUMO-Targets-and-Temporal-Regulation-of-SUMOylation-of-pone.0103308.g002.jpg

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English: (A) 293T cells in six-well plates were cotransfected with 0.5 µg of plasmid expressing myc-US34A, myc-UL150, flag-SUMO-1, or flag-SUMO-2 as indicated. At 48 h, total cell lysates were prepared and immunoblotted with an anti-myc antibody. The bands corresponding to unmodified and SUMO-modified forms of myc-US34A and unmodified myc-UL150 are indicated. NS, non-specific bands. (B) In vitro SUMOylation reactions. Myc-UL123(IE1) and myc-US34A produced by in vitro transcription/translation were incubated with GST-SAE2/1, His-Ubc9, and His-SUMO-1GG or GST-SUMO-1GG as indicated. The reaction products were analyzed by SDS-PAGE (8%) and immunoblot assays with a myc-IE1 antibody. Unmodified and SUMO-modified forms of IE1 and US34A are indicated. (C) The disorder in UL122 (IE2), UL123 (IE1), and US34A was predicted with the IUPred program (http://iupred.enzim.hu). The lysine residues modified by SUMO (for IE1 and IE2) or predicted to be SUMOylation sites (for US34A) are indicated.
Date
Source Image file from Kim E, Kim Y, Kim Y, Lee M, Hayward G, Ahn J (2014). "Analysis of Human Cytomegalovirus-Encoded SUMO Targets and Temporal Regulation of SUMOylation of the Immediate-Early Proteins IE1 and IE2 during Infection". PLOS ONE. DOI:10.1371/journal.pone.0103308. PMID 25050850. PMC: 4106884.
Author Kim E, Kim Y, Kim Y, Lee M, Hayward G, Ahn J
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