File:Analysis-of-Human-Cytomegalovirus-Encoded-SUMO-Targets-and-Temporal-Regulation-of-SUMOylation-of-pone.0103308.g006.jpg

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Analysis-of-Human-Cytomegalovirus-Encoded-SUMO-Targets-and-Temporal-Regulation-of-SUMOylation-of-pone.0103308.g006.jpg ‎(685 × 450 pixels, file size: 53 KB, MIME type: image/jpeg)

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English: (A) In vitro SUMOylation reactions for IE1 were conducted as in Fig. 5C using His-IE1, GST-SAE2/1, His-Ubc9, GST-SUMO-1GG, and increasing amounts (0.1, 0.5, and 1 µg) of GST or GST-IE2(346–542) with (left panel) or without (right panel) immunoprecipitated flag-PIAS1. The reaction products were analyzed by SDS-PAGE (8%) and immunoblot assays with anti-IE1 antibody. (B) The GST and GST-IE2(346–542) proteins added to in vitro SUMOylation reactions were detected by immunoblotting with an anti-GST antibody.
Date
Source Image file from Kim E, Kim Y, Kim Y, Lee M, Hayward G, Ahn J (2014). "Analysis of Human Cytomegalovirus-Encoded SUMO Targets and Temporal Regulation of SUMOylation of the Immediate-Early Proteins IE1 and IE2 during Infection". PLOS ONE. DOI:10.1371/journal.pone.0103308. PMID 25050850. PMC: 4106884.
Author Kim E, Kim Y, Kim Y, Lee M, Hayward G, Ahn J
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