Asymmetric distribution of cyb-3 in 4-cell stage embryos
03/21/2107 – Open Access
Asymmetric distribution of cyb-3 in 4-cell stage embryos
W. Matthew Michael1
1Molecular and Computational Biology Section, Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, United States of America.
Description:
Early embryos were fixed and stained with Mab F2F4 (green), shown to recognize CYB-3 (Michael, 2016), and DAPI, to illuminate the DNA (blue). Either wild type or par-4 mutant embryos were examined, after 24-hour incubation at 25C (the non-permissive temperature for the it47 allele of par-4). Anterior is to the left in all images. The data presented here reveals previously not shown data that depicts
CYB-3 as asymmetrically distributed at the 4-cell stage. These data further support reported findings in
Michael, 2016. There is more CYB-3 in the AB cell relative to its sister P1. In 4-cell embryos there is more CYB-3 in the EMS cell relative to its sister, P2. Thus, during P-lineage divisions, CYB-3 is asymmetrically distributed such that the somatic precursor receives more than its germline precursor sister cell. This asymmetry is abolished in par-4 mutant embryos, where all blastomeres contain
equivalent amounts of CYB-3.
References
Michael WM. Cyclin CYB-3 controls both S-phase and mitosis and is asymmetrically distributed in the early C. elegans embryo. Development. 2016 Sep 1;143(17):3119-27. doi: 10.1242/dev.141226. PubMed PMID: 27578178; PubMed Central PMCID: PMC5047676.
Reagents
Antibody: Mab F2F4
Strain: KK184 par-4(it47ts) V
National Institute of Health R01 GM099825 grant
Reviewed by: Ian Hope
Received 03/15/2017, Accepted 03/16/2017. Available starting WormBase release WS260, Published Online 03/21/2017.
Copyright: © 2017. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Michael, WM. (2017): Asymmetric distribution of cyb-3 in 4-cell stage embryos. WormBase. Dataset. https://doi.org/10.17912/W27P4P.
This work is released under the Creative Commons Attribution 4.0 International license, which allows free use, distribution, and creation of derivatives, so long as the license is unchanged and clearly noted, and the original author is attributed.
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