connect the interior space with the atmosphere. The tubes are bent several times up and down, so as to intercept and retain the particles carried by such feeble currents as changes of temperature might cause to set in between the outer and the inner air.
The bottom of the box is pierced sometimes with a single row sometimes with two rows of holes, in which are fixed, air-tight, large test-tubes, intended to contain the liquid to be exposed to the action of the moteless air.
On the 10th of September the first case of this description was closed. The passage of a concentrated beam across it through its two side-windows then showed the air within it to be laden with floating matter. On the 13th it was again examined. Before the beam entered, and after it quitted the case, its track was vivid in the air, but within the case it vanished. Three days of quiet sufficed to cause all the floating matter to be deposited on the sides and bottom, where it was retained by a coating of glycerine, with which the interior surface of the case had been purposely varnished. The test-tubes were then filled through the pipette, boiled for five minutes in a bath of brine or oil, and abandoned to the action of the moteless air.
During ebullition, aqueous vapor rose from the liquid into the chamber, where it was for the most part condensed, the uncondensed portion escaping, at a low temperature, through the bent tubes at the top. Before the brine was removed, little stoppers of cotton-wool were inserted in the bent tubes, lest the entrance of the air into the cooling chamber should at first be forcible enough to carry motes along with it. As soon, however, as the ambient temperature was assumed by the air within the case, the cotton-wool stoppers were removed.
We have here the oxygen, nitrogen, carbonic acid, ammonia, aqueous vapor, and all the other gaseous matters which mingle more or less with the air of a great city. We have them, moreover, "untortured" by calcination, and unchanged even by filtration or manipulation of any kind. The question now before us is, can air thus retaining all its gaseous mixtures, but self-cleansed from mechanically suspended matter, produce putrefaction? To this question, both the animal and vegetable worlds return a decided negative. Among vegetables, experiments have been made with hay, turnips, tea, coffee, hops, repeated in various ways with both acid and alkaline infusions. Among animal substances are to be mentioned many experiments with urine; while beef, mutton, hare, rabbit, kidney, liver, fowl, pheasant, grouse, haddock, sole, salmon, cod, turbot, mullet, herring, whiting, eel, oyster, have been all subjected to experiment.
The result is, that infusions of these substances exposed to the common air of the Royal Institution laboratory, maintained at a temperature of from 60° to 70° Fahr., all fell into putrefaction in the course of from two to four days. No matter where the infusions
