File:Analysis-of-Human-Cytomegalovirus-Encoded-SUMO-Targets-and-Temporal-Regulation-of-SUMOylation-of-pone.0103308.g007.jpg
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Analysis-of-Human-Cytomegalovirus-Encoded-SUMO-Targets-and-Temporal-Regulation-of-SUMOylation-of-pone.0103308.g007.jpg (792 × 350 pixels, file size: 55 KB, MIME type: image/jpeg)
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DescriptionAnalysis-of-Human-Cytomegalovirus-Encoded-SUMO-Targets-and-Temporal-Regulation-of-SUMOylation-of-pone.0103308.g007.jpg |
English: (A and B) The reporter assays using the ISG54 ISRE-luciferase construct. 293T cells in 12-well plates were cotransfected with 0.5 µg of the ISG54 ISRE-luciferase reporter construct and plasmids expressing HA-IE1, flag-SUMO-1, HA-PIAS1, and myc-IE2(346–542) as indicated. The total amount of plasmid was adjusted with empty vectors. At 24 h, cells were untreated or treated with IFNβ (1,000 units/ml) for 8 h, and luciferase reporter assays were performed. The results shown are the mean values and standard errors of three independent experiments. Statistical significance between samples was determined using Student's t-test (values of *P<0.0005). The expression levels of IE1, IE2, and β-actin proteins in cell lysates were determined by immunoblotting with specific antibodies. (C) A hypothetical model showing that expression of IE2 and its SUMOylation regulates the PIAS1-mediated IE1 SUMOylation, enhancing IE1 activity to downregulate type I IFN-stimulated gene (ISG) expression. ISRE, interferon stimulated response element. |
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Source | Image file from Kim E, Kim Y, Kim Y, Lee M, Hayward G, Ahn J (2014). "Analysis of Human Cytomegalovirus-Encoded SUMO Targets and Temporal Regulation of SUMOylation of the Immediate-Early Proteins IE1 and IE2 during Infection". PLOS ONE. DOI:10.1371/journal.pone.0103308. PMID 25050850. PMC: 4106884. | |
Author | Kim E, Kim Y, Kim Y, Lee M, Hayward G, Ahn J | |
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 02:44, 25 July 2014 | 792 × 350 (55 KB) | Recitation-bot | Automatic upload of media from: doi:10.1371/journal.pone.0103308 |
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Publisher | Public Library of Science |
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Source | info:doi/10.1371/journal.pone.0103308 |
Date(s) | 22 July 2014 |
Identifier | info:doi/info:doi/10.1371/journal.pone.0103308.g007 |
IIM version | 2 |