03/21/2107 – Open Access
Asymmetric distribution of cyb-3 in 4-cell stage embryos
W. Matthew Michael1
1Molecular and Computational Biology Section, Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, United States of America.
Description:
Early embryos were fixed and stained with Mab F2F4 (green), shown to recognize CYB-3 (Michael, 2016), and DAPI, to illuminate the DNA (blue). Either wild type or par-4 mutant embryos were examined, after 24-hour incubation at 25C (the non-permissive temperature for the it47 allele of par-4). Anterior is to the left in all images. The data presented here reveals previously not shown data that depicts
CYB-3 as asymmetrically distributed at the 4-cell stage. These data further support reported findings in
Michael, 2016. There is more CYB-3 in the AB cell relative to its sister P1. In 4-cell embryos there is more CYB-3 in the EMS cell relative to its sister, P2. Thus, during P-lineage divisions, CYB-3 is asymmetrically distributed such that the somatic precursor receives more than its germline precursor sister cell. This asymmetry is abolished in par-4 mutant embryos, where all blastomeres contain
equivalent amounts of CYB-3.
References
Michael WM. Cyclin CYB-3 controls both S-phase and mitosis and is asymmetrically distributed in the early C. elegans embryo. Development. 2016 Sep 1;143(17):3119-27. doi: 10.1242/dev.141226. PubMed PMID: 27578178; PubMed Central PMCID: PMC5047676.
Reagents
Antibody: Mab F2F4
Strain: KK184 par-4(it47ts) V
Funding:
National Institute of Health R01 GM099825 grant
