Page:Bergey's manual of determinative bacteriology.djvu/1032

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1010
COMPREHENSIVE KEY

Note: The descriptions given for species in this genus are like early descriptions given for Arthrobacler and in some instances could fit Nocardia. Not as above 18 18. Organisms disintegrate filter paper in 0.5 per cent peptone water; produce clearing on precipitated cellulose agar plates ; motile (except C. flavigena) Cellulomonas p. 601 Not as above possibly Corynebacterium p. 579 19. Motile at 37° or at 25°C 20 Non-motile 27 20. Organisms pathogenic to warm-blooded animals causing monocytosis; catalase-posi- tive; acid produced from glucose, salicin and aesculin Listeria -p. 597 Not as above 21 21. Pathogenic to plants 22 Not pathogenic to plants 24 22. Yellow colonies; polar flagella possibly Corynebacterium -p. 579 (C. flaccumfaciens or C. tritici) Or. . . . Xanthonionas p. 152 {X. proteamaculans or X. conjac) Yellow colonies; peritrichous flagella Erwinia p. 349 {E. citrimaculans) Colonies not yellow 23 23. Flagella polar Corynebacterium p. 579 (C. poinsettiae or C. hypertropMcans) Or ... . Pseudomonas p . 89 (P. polygoni) Flagella peritrichous Erwinia p. 34.9 {E. carnegieana) Note: Erwinia species are generally regarded as Gram-negative. Gram-variable species are recorded. 24. Organisms disintegrate filter paper in 0.5 per cent peptone water; produce clearing on precipitated cellulose agar plates Cellulomonas p. 601 Not as above 25 25. No acid from carbohydrates 26 Acid from carbohydrates Brevibacterium p. 490 (Including Flavobacterium suaveolens and F. marinum) 26. Organisms occur in long chains; colonies colorless and spreading. . . . Kurthia p. 503 Organisms arranged singly; colonies yellow Brevibacterium p. 490 (B. suljureum) 27. Acid-fast Mycobacterium p. 695 Not acid-fast 28 28. Organisms 0.2 to 0.4 by 0.5 to 2.5 microns; long filaments common in rough colonies; occur singly and also in chains; pinpoint transparent colonies on agar in 24 hours at 37 °C., extending on further incubation to 1.5 mm; acid only produced from glucose and lactose and some other carbohydrates but not from aesculin; final pH in glucose broth, about 6.0; hydrogen sulfide is produced; causes swine erysipelas, human erysipeloid, mouse septicemia and infections in sheep, birds and fish Erysipelothrix p. 599 Not as above 29 29. Rods 0.4 to 0.7 by 1 to 3 microns; show granular staining with methylene blue; arranged in angular fashion similar to the corynebacteria; only 2 species recorded, both of which produce acid from glucose, fructose and mannose; catalase-positive; resist heating to 72°C. for 15 minutes; normally found in dairy products and equipment Microbacterium p. 600 Not as above 30