kauer negative; starch hydrolyzed ; pathogenic to the fish Salmonidae Aeromonas p. 189 Not as above : Voges-Proskauer positive 76 76. Organisms encapsulated; cause respiratory and other infections in man Klebsiella p. 3U Not as above Paracolobactruni p. 346 (P. aerogenoides strains 32011 and 32811) 77. Obligate halophiles requiring 20 to 30 per cent salt for growth; pleomorphic; yellow colonies Halobacteriuin p. 207 Not as above 78 78. Organisms will not grow' on meat infusion agar without the addition of blood or ascitic fluid or X and V factors or other enrichments 79 Not as above 80 79. Pleomorphic coccobacillary organisms; grow well on blood agar producing colonies up to 4 mm in diameter on prolonged incubation; hydrogen sulfide produced from cystine media; slight acid from glucose, fructose and mannose and possibly from other sugars. Pathogenic, producing tularemia or tularemia-like infections in laboratory animals Pasteurella p. 395 Organisms usually 0.2 to 0.5 micron wide and 0.5 to 2.0 microns long but frequently produce long filaments; do not grow on nutrient agar or on MacConkey's lactose bile salt agar; grow on nutrient agar with the addition of X factor or V factor or both or with the addition of diphosphothiamine or adenosine triphosphate; colonies on suit- able media rarely more than 1 mm in diameter after 2 days incubation; nitrites produced from nitrates; various species have been reported as responsible for or associated with viruses in: (i) Purulent meningitis and conjunctivitis in man (H. influenzae) (ii) Sub-acute endocarditis {H. hemolyticus) (iii) Acute and sub-acute conjunctivitis in man (H. aegyptius) (iv) Soft chancre {H. ducreyi) (v) Vesicular eruptions in the genitals of cattle {H. citreus) (vi) Ulcers of trout {H. piscium) (vii) Commonly present in the respiratory tract of man (various species) and preputial secretions of dogs {H. haemoglobinophilus) (viii) Pharyngitis {H. parahaemolyticus) (ix) Non-pathogenic {H. parainfluenzae) (x) Fowl coryza {H. gallinarum) (xi) Swine influenza {H. suis) (xii) Respiratory tract of ferrets {H. putoriorum) Haemophilus p. ^06 Note: This is the main entry for the genus Haemophilus. Owing to variable biochemical characteristics, other entries occur in other parts of the key. Fastidious organisms; require blood or ascitic fluid; highly pleomorphic; rod-shaped or filamentous; may reach 100 to 150 microns in length; homogeneous in young cul- tures but become granulated, beaded and swollen in old cultures and tend to frag- ment into rods and cocci; large spherical swellings packed with granules common; cholesterol globules appear among the growth; only rods and short filaments appear in pathological material; non-branching; non-acid fast; colonies on ascitic agar 1 to 2.5 mm in 3 days; circular, low convex, colorless. L-phase variants are common. Highly virulent for mice causing polyarthritis; causes rat-bite or Haverhill fever in man Streptobacillus p. 451 {S. moniliformis) 80. Slender rods; 1.0 to 3.0 microns long and often arranged in angular fashion; pleo- morphic; produce smooth, entire, butyrous, translucent, grayish yellow colonies 0.5 to 1.0 mm in diameter in 2 days at 37 °C. on meat infusion agar; may increase
