86. Agar digested Agarbacterium p. 322 Agar not digested 87 87. Colonies yellow 88 Colonies not yellow 92 88. Organisms will grow in a mineral salts medium using CO2 as a source of carbon and oxidizing hydrogen Hydrogenomonas p. 75 Organisms grow in a mineral salts medium using alkylamines as the sole source of carbon and nitrogen Protaminobacter p. 200 Not as above 89 89. Obligate halophiles requiring 20 to 30 per cent salt for growth Halobacteriiim p. 207 Not as above 90 90. Litmus milk acid or unchanged Flavobacterium p. 309 Litmus milk alkaline 91 91. Nitrites produced from nitrates Flavobacterium p. 309 (F. lutescens and F. fucatum) Nitrites not produced from nitrates Alcaligenes p. 297 {A. marshallii) 92. Loeffler's inspissated serum liquefied 93 Not as above 9^ 93. Recorded from septicemia in ducks Pasteiirella p. 395 Cause acute ophthalmia (pink eye) of cattle; angular conjunctivitis in man, monkeys and apes and recorded as a possible cause of trachoma in man Moraxella p. ^79 94. Organisms recorded from an enzootic of chronic pneumonia in calves; thin Gram- negative rods arranged in groups in tissues and as coccoid and bacillary forms in culture; in the condensation water of blood serum, produce mulberry -like flakes up to 1 mm in diameter consisting of cells arranged in chains, the latter encased in a non-stainable material which usually is swollen at the tip Actinobacillus p. 4H (A. aciinoides) Not as above 95 95. Slender rods; 1.0 to 3.0 microns long, often arranged in angular fashion; pleomorphic; produce smooth, entire, butyrous, translucent, grayish yellow colonies 0.5 to 1.0 mm in diameter in 2 days at 37° C. on meat infusion agar; may increase slightly in size on further incubation; cafe au lait-coored on potato; Straus reaction produced in guinea pigs; strict parasite causing glanders in man and other animals Actinobacillus p. 4H {A. mallei) Not as above 96 96. Organisms cause brucellosis in man and other animals; often cause abortion in ani- mals; good growth on liver extract agar or tryptose phosphate agar; increased CO2 tension necessary for isolation of one species; litmus milk alkaline Brucella p. 404 Not as above 97 97. Organisms causing whooping cough in man, principally in children. One species {B. pertussis) will not grow on primary isolation on meat extract agar but does not require X or V factor; the other species {B. parapertussis) grows profusely on meat extract agar. Both species grow well on Bordet-Gengou medium producing smooth, raised, entire, pearly, glistening colonies in 48 to 72 hours at 37° C; nitrites not produced from nitrates; inspissated serum not liquefied. Following intraperitoneal injection into mice, death occurs in 2 to 4 days. Autopsj^ shows extensive hyperemia of the peritoneal wall, infiltration of glands and the presence of an extremely mucoid
