106. Flagella polar; gelatin liquefied; some species are pathogenic to fish, frogs and snakes while others are saprophytic Aeromonas p. 189 (See also Pseudomonas polygoni and P. colurnae (if lactose is not fermented).) Flagella peritrichous 107 107. Gelatin liquefied or indole produced or both; organisms may show a marked spreading growth on solidified agar with a film of moisture on the surface .... Proteus p. 364 (See also Paracolobactrum calif orme strains 4361 and 33811 and Providence Group, Rome, 1953, for aerogenic strains.) Note: Proteus spp. are probably also urease-positive. The character is not mentioned in Manual descriptions. Gelatin is not liquefied and indole is not produced; colonies do not spread on solidified agar 108 108. Voges-Proskauer positive Paracolobactrum p. 34-6 (P. aerogenoidss strains 32011, 37711, 35611, 37211, 37511 and 32821) Voges-Proskauer negative Salmonella p. 368 (Salmonella Group, Rome, 1953) (See also Paracolobactrum intermedium strains 12611 and 1421 and non-lactose- fermenting strains of the Bethesda-Ballerup Group, Rome, 1953.) 109. Colonies yellow 110 Colonies not yellow 113 110. Bioluminescent on media containing 3 per cent salt Photobacterium p. 193 Not as above Ill 111. Chitin is hydrolyzed Beneckea p. 328 Not as above 112 112. Flagella polar Xanthomonas p. 152 (See Pseudomonas xantha and P. piciorum and also P. Irifolii, P. radiciperda and Bacterium tardicrescens if they produce no acid from lactose.) Flagella peritrichous Flavobacterium p. 309 Note: The following plant pathogens also terminate at this point: Erwinia cassavae, E. citrimaculans and Agrobacterium gypsophilae. 113. Animal parasites; colonies mucoid; isolated from the eye of the Rhesus monkey Noguchia p. 421 Small translucent colonies; mucoid, small slender rods in smooth colonies; ovoid rods with bipolar staining in rough colonies; motile at 37° C; organisms cause meliodio- sis in man and other animals Pseudomonas p. 89 (P. pseudomallei) Note: This insertion covers strains which have lost their ability to produce acid from lactose. Small umbonate colonies, translucent with a dull finely granular "beaten copper" surface, entire, butyrous; organisms cause pseudotuberculosis in rodents Pasteurella p. 395 Organisms found in the cytoplasm of the rhizopod Pelomijxa palustris Myxococcus p. 930 (M. pelomyxae) Not as above 114 114. Organisms cause typhoid fever in man; agglutinated by Salmonella group D "O" antiserum when not in the "Vi" phase; flagella peritrichous Salmonella p. 368 (S. typhosa) Not as above 115 115. Agar is digested Agarbacterium p. 322 Agar is not digested 116
