Page:Bergey's manual of determinative bacteriology.djvu/130

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108
ORDER I. PSEUDOMONADALES

Nat. Acad. Sci., 8, 1896, 22; Chester, Man. Determ. Bact., 1901, 325.)

stri.a′ta. L. v. strio to groove; L. part. adj. striatus grooved.

Slender rods, of variable lengths, staining irregularly, occurring singly and in pairs. Motile, possessing polar flagella. Gram-negative.

Gelatin colonies: Circular, yellowish, with filamentous border.

Gelatin stab: No liquefaction.

Agar slant: Smooth, glistening, irregular, spreading. Agar becomes yellowish green.

Broth: Turbid, becoming slightly greenish.

Litmus milk: No coagulation; becoming alkaline; litmus reduced.

Potato: Moist, glistening, spreading, becoming chocolate-brown.

Indole not produced.

Aerobic.

Grows well at 25° and 36° C.

Source: Isolated from soil.

Habitat: Soil.


22. Pseudomonas ovalis Chester, 1901. (Bacillus fluorescens ovalis Ravenel, Memoirs Nat. Acad. Sci., 8, 1896, 9; Chester, Man. Determ. Bact., 1901, 325.)

o.va′lis. L. n. ovum an egg; M.L. adj. ovalis oval.

Rods, short with rounded ends, occurring singly. Motile, possessing polar flagella. Gram-negative.

Gelatin colonies: Irregular, lobate, slightly granular, translucent, grayish becoming bluish.

Gelatin stab: No liquefaction. Faintly green near surface.

Agar colonies: Circular, opaque, entire, greenish fluorescence.

Agar slant: Thin, spreading, greenish white. Agar becomes yellowish.

Broth: Turbid, with pellicle and white sediment; faintly green.

Potato: Scant, yellowish brown growth.

Indole not produced.

Aerobic, facultative.

Grows well at 25° and 36° C.

Source: Isolated from soil.

Habitat: Soil.


23. Pseudomonas taetrolens Haynes, nom. nov. (Pseudomonas graveolens Levine and Anderson, Jour. Bact., 23, 1932, 343; not Pseudomonas graveolens Migula, Syst. d. Bakt., 2, 1900, 934.)

taet′ro.lens. L. adj. taeter offensive; L. part, olens having an odor; M.L. part. adj. taetrolens foul-smelling.

Short rods with rounded ends, occurring singly, in pairs and in short chains. Motile (Levine and Anderson). One to five polar flagella (found on retest of cultures by Haynes, 1953). Gram-negative.

Gelatin stab: Not liquefied.

Agar colonies: Circular, slightly raised, smooth, entire, amorphous internal structure.

Agar slant: Growth abundant and tan-colored; medium darkened. Penetrating odor of must. Broth: Turbid; thin, oily pellicle and sediment. Odor of must.

Litmus milk: Acid, coagulated; litmus reduced.

Indole not produced.

Nitrites not produced from nitrates.

Hydrogen sulfide produced.

Starch not hydrolyzed.

Acid but no gas produced from glucose, lactose, galactose, mannose, fructose, rhamnose and xylose. Slight acidity in glycerol and mannitol. No acid or gas from aesculin, amygdalin, arabitol, dextrin, dulcitol, glycogen, inulin, maltose, melizitose, pectin, raffinose, salicin, sorbitol, starch, sucrose, xylan, arabinose, erythritol or trehalose.

Aerobic.

Catalase-positive.

Optimum temperature, between 23° and 25° C. Scant growth at 33° and 10° C.

Distinctive character: A strong musty odor develops in media in which this organism grows. In this respect it resembles P. perolens which, however, liquefies gelatin and reduces nitrates.

Source: Isolated from musty eggs; also from milk by Olsen and Hammer (Iowa State College Jour. Sci., 9, 1934, 125).

Habitat: Found in various foods that have a musty odor; presumably widely distributed.


24. Pseudomonas incognita Chester, 1901. (Bacillus fluorescens incognitus Wright,