439; Chester, Man. Determ. Bact., 1901, 308.)
am.bi′gu.a. L. adj. ambiguus going about, hence uncertain.
Small rods, with rounded ends, occurring singly, in pairs and in chains. Motile, possessing a polar flagellum. Gram-negative.
Gelatin colonies: Gray, translucent, slightly raised, irregular, radiate, with transparent margin.
Gelatin stab: No liquefaction.
Agar slant: Gray, limited, entire.
Broth: Turbid, with gray sediment.
Litmus milk: Acid, slowly coagulated. Litmus reduced.
Potato: Gray to creamy, viscid, spreading.
Indole produced.
Aerobic, facultative.
Optimum temperature, between 30° and 35° C.
Source: Isolated from water from the Schuylkill River.
Habitat: Water.
36. Pseudomonas oleovorans Lee and Chandler, 1941. (Jour. Bact., 41, 1941, 378.)
o.le.o′vor.ans. L. oleum oil; L. v. voro to destroy, consume; M.L. part. adj. oleovorans oil-consuming.
Short rods, 0.5 by 0.8 to 1.5 microns, occurring singly and in pairs. Motile. Gram-negative.
Gelatin stab: No liquefaction after 6 weeks.
Gelatin colonies: Up to 1 mm in diameter, fluorescent; similar to agar colonies.
Surface agar colonies: After 24 hours 1 to 2 mm in diameter, smooth, convex, shiny, opaque, creamy, fluorescent by transmitted light. Edge entire in young colonies.
Deep agar colonies: 0.5 by 1.0 to 1.5 mm, lens-shaped, buff-colored, not fluorescent.
Agar slant: Growth raised, smooth, fluorescent, edge erose.
Broth: After 24 hours, moderate turbidity with slight yellowish, viscid sediment. No pellicle or ring. No soluble pigment produced.
Litmus milk: No change.
Indole not produced.
Potato: Good growth.
Nitrites are produced from nitrates.
Starch is hydrolyzed.
No acid from glucose, lactose, sucrose, galactose, xylose, mannitol, salicin or glycerol.
Equally good growth at 25° and 37° C.
Aerobic.
Distinctive character: The fluorescent quality of the colonies is not imparted to any of the artificial media used.
Source: Isolated from cutting compound (oil-water emulsion) circulating in a machine shop. The oil in this compound may be utilized as a sole source of energy.
Habitat: Probably oil-soaked soils. Abundant in cutting compounds.
37. Pseudomonas arvilla Gray and Thornton, 1928. (Cent. f. Bakt., II Abt., 73, 1928, 90.)
ar.vil′.la. L. arvum a field; M.L. dim. noun arvilla a small field.
Rods, 0.5 to 0.7 by 2.0 to 3.0 microns. Motile with one to five polar flagella. Gram-negative.
Gelatin colonies: Circular, whitish, convex, smooth, glistening, lobate.
Gelatin stab: No liquefaction.
Agar colonies: Circular or amoeboid, white to buff, flat to convex, smooth, glistening, opaque, entire.
Agar slant: Filiform, whitish, convex, smooth, ringed, entire.
Broth: Turbid.
Nitrites not produced from nitrates.
Starch not hydrolyzed.
Acid from glucose.
Attacks naphthalene.
Aerobic, facultative.
Grows at 37° C.
Source: Isolated from soil.
Habitat: Soil.
38. Pseudomonas dacunhae Gray and Thornton, 1928. (Cent. f. Bakt., II Abt., 73, 1928, 90.)
da.cun′hae. d'Acunha, place name. Island; M.L. gen. noun dacunhae of d'Acunha.
Rods 0.5 to 0.8 by 1.5 to 3.0 microns. Motile with one to six polar flagella. Gram-negative.
Gelatin colonies: Circular, whitish, raised, smooth, glistening, entire.
Gelatin stab: No liquefaction.
