Page:Bergey's manual of determinative bacteriology.djvu/160

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138
ORDER I. PSEUDOMONADALES

Habitat: Causes a root rot of ginseng, Panax quinquefolium.


107. Pseudomonas ribicola Bohn and Maloit, 1946. (Jour. Agr. Res., 73, 1946, 288.)

ri.bi'co.la. M.L. noun Ribes generic name of currant; L. colo to dwell; M.L. fem.n. ribicola the currant dweller.

Rods, 0.4 to 0.9 by 0.9 to 1.7 microns, occurring singly, in pairs and in hypha-like chains. Motile by 1 or more polar flagella. Gram-negative.

Gelatin: Very slow liquefaction.

Beef-extract agar colonies: Punctiform, smooth, translucent, white; edges entire.

Beef-extract agar slant: Growth scant, filiform, glistening, translucent, white, slightly viscid.

Broth: Slightly turbid; no ring or pellicle.

Potato dextrose slants: Growth moderate, filiform, glistening, butyrous to viscid. Medium slightly yellow. Dirty pink pigment in old cultures.

Milk: Slightly darkened, becoming alkaline.

Nitrites produced from nitrates.

Growth good in Uschinsky's and Fermi's solutions; yellow-green pigment produced. No growth in Cohn's and Ashby's mannitol solutions.

Indole not produced.

Hydrogen sulfide not produced.

Acid from glucose, galactose, fructose, xylose and mannitol.

Starch not hydrolyzed.

Asparagine utilized as a carbon-nitrogen source. Tyrosine oxidized.

Not lipolytic.

Temperature relations: Optimum, between 20° and 25° C. Minimum, less than 3.5° C. Maximum, between 30° and 32° C.

Source: Six single-cell isolates from leaf spot of golden currant in Wyoming.

Habitat: Pathogenic on Ribes aureum.


108. Pseudomonas xanthochlora (Schuster, 1912) Stapp, 1928. (Bacterium xanthochlorum Schuster, Arbeit, a. d. Kaiserl. Biolog. Anstalt. f. Land. u. Forstw., 8, 1912, 452; Stapp, in Sorauer, Handbuch der Pflanzenkrankheiten, 2, 5 Aufl., 1928, 213.)

xan.tho.chlō'ra. Gr. xanthus yellow; Gr. chlorus green; M.L. adj. xanthochlorus yellowish green.

Description from Erw. Smith (Bacteria in Rel. to Plant Dis., 3, 1914, 272).

Rods 0.75 to 1.5 by 3.0 microns. Motile with 1 to 3 polar flagella. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Slow liquefaction.

Agar colonies: Circular, slightly raised, yellow-white.

Broth: Strong clouding in 24 hours. A white pellicle.

Milk: Slow coagulation and clearing.

Nitrites produced from nitrates.

Indole produced after 10 days.

Hydrogen sulfide produced slowly.

Acid but no gas from glucose and galactose.

Temperature relations: Optimum, 27° C. Minimum, 2° C. Maximum, 44° C.

Source: Isolated from rotting potato tubers in Germany.

Habitat: Pathogenic on potato tubers and a number of unrelated plants.


109. Pseudomonas aleuritidis (McCulloch and Demaree, 1932) Stapp, 1935. (Bacterium aleuritidis McCulloch and Demaree, Jour. Agr. Res., 45, 1932, 339; Stapp, Bot. Rev., 1, 1935, 408.)

a.leu.ri'ti.dis. Gr. aleurites of wheaten flour; M.L. fem.noun Aleurites generic name; M.L. gen.noun aleuritidis of Aleurites.

Rods 0.6 to 0.7 by 1.1 to 3.0 microns. Motile with 1 to 5 polar, rarely bipolar, flagella. Encapsulated. Gram-negative.

Green fluorescent pigment produced in certain media.

Gelatin: Not liquefied.

Beef agar slants: Growth is thin, white and viscid.

Broth: A heavy white surface growth in 24 hours. Sediment.

Milk: Becomes alkaline, but no separation.

Nitrites produced from nitrates.

Indole test feebly positive.

Hydrogen sulfide test feebly positive.

Acid but no gas from glucose, galactose and glycerol. Slow acid production from sucrose, maltose and lactose.