Gelatin: No liquefaction.
Potato glucose agar colonies: Flat, circular, shining; margins somewhat undulated.
Broth: Dense clouding with partial pellicle.
Milk: Alkaline. No separation.
Nitrites not produced from nitrates (Adam and Pugsley).
Indole produced. Not produced (Adam and Pugsley).
Acid but no gas from glucose and sucrose. No acid from lactose (Adam and Pugsley).
Starch not hydrolyzed (Adam and Pugsley).
Comment: Pseudomonas savastanoi is similar in culture but is not pathogenic on oleanders.
Source: Both Ferraris and C. O. Smith isolated the pathogen from galls on oleander.
Habitat: Pathogenic on oleander, Nerium oleander.
113. Pseudomonas cissicola (Takimoto, 1939) Burkholder, 1948 (Aplanobacter cissicola Takimoto, Ann. Phytopath. Soc. Japan, 9, 1939, 43; Burkholder, in Manual, 6th ed., 1948, 134.)
cis.si'co.la. Gr. cissus ivy; M.L. fem.noun Cissus generic name of flowering plant; L. -cola dweller; M.L. fem.noun cissicola Cissus dweller.
Rods 0.5 to 0.9 by 1.0 to 2.0 microns. Non-motile. Encapsulated. Gram-negative.
Green fluorescent pigment formed in Uschinsky's solution.
Gelatin: No liquefaction.
Potato-extract agar colonies: Circular, convex, smooth, dirty white.
Broth: Feeble clouding followed by precipitation of pellicle and rim.
Nitrites not produced from nitrates.
Indole not produced.
Hydrogen sulfide not produced.
No acid nor gas from sucrose, glucose, lactose or glycerol.
Starch not hydrolyzed.
Salt toleration, 3 per cent.
Temperature relations: Optimum, 30° C. Minimum, 10° C. Maximum, 35° C. Thermal death point between 49° and 50° C.
Source: Isolated from black spots on leaves of Japanese ivy, Cissus japonica, in Japan.
Habitat: Pathogenic only on Cissus japonica.
114. Pseudomonas calendulae (Takimoto, 1936) Dowson, 1943. (Bacterium calendulae Takimoto, Ann. Phytopath. Soc. Japan, 5, 1936, 341; Dowson, Trans. Brit. Mycol. Soc, 26, 1943, 9.)
ca.len'du.lae. L. fem.pl.noun calendae (Kalendae) The Calends, first day of month; M.L. fem.dim.noun Calendula generic name of a flowering plant; M.L. gen.noun calendulae of Calendula.
Rods 0.5 by 1.0 to 2.0 microns. Motile with 1 to 3 polar flagella. Gram-negative.
Green fluorescent pigment produced in Uschinsky's and in Cohn's solutions.
Gelatin: Not liquefied.
Agar colonies: Circular, smooth, flat, dirty white.
Broth: Turbid.
Milk: No coagulation.
Nitrites not produced from nitrates.
Indole produced in small amount.
Hydrogen sulfide not produced.
Acid but no gas from glucose and glycerol. No acid from lactose or sucrose.
Starch not hydrolyzed.
Temperature relations: Optimum between 27° and 30° C. Minimum between 0° and 7° C. Maximum, 37° C.
Habitat: Pathogenic on marigolds, Calendula officinalis.
115. Pseudomonas cichorii (Swingle, 1925) Stapp, 1928. (Phytomonas cichorii Swingle, Phytopath., 15, 1925, 730; Stapp, in Sorauer, Handbuch der Pflanzenkrankheiten, 2, 5 Aufll., 1928, 291; Pseudomonas endiviae Kotte, Phyt. Ztschr., 1, 1930, 609; Bacterium formosanum Okabe, Jour. Soc. Trop. Agr., Formosa, 7, 1935, 65.)
ci.cho'ri.i. Gr. cichora (pl.) succory, chicory; L. cichorium chicory; M.L. neut.noun Cichorium generic name of flowering plant; M.L. gen.noun cichorii of chicory.
Description from Clara (Cornell Agr. Exp. Sta. Mem. 159, 1934, 26) which is a description of a culture of Pseudomonas endiviae from Kotte. Swingle's description is very meager.
