Page:Bergey's manual of determinative bacteriology.djvu/164

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142
ORDER I. PSEUDOMONADALES

Aerobic.

Source: Isolated from angular leaf spots and stem lesions on arrow-wood, Viburnum opulus, etc.

Habitat: Pathogenic on Viburnum spp.


118. Pseudomonas mori (Boyer and Lambert, 1893) Stevens, 1913. (Bacterium mori Boyer and Lambert, Compt. rend. Acad. Sci., Paris, 117, 1893, 342; Bacterium mori Boyer and Lambert emend. Erw. Smith, Science, 31, 1910, 792; Stevens, The Fungi which Cause Plant Diseases, 1913, 30.)

mo'ri. Gr. morum the black mulberry; M.L. fem.noun Morus the generic name of mulberry; M.L. gen.noun mori of the mulberry.

Description from Smith (op. cit., 1910, 792).

Rods 0.9 to 1.3 by 1.8 to 4.5 microns. Motile with a polar flagellum. Gram-negative.

Green fluorescent pigment produced in culture.

Gelatin: Not liquefied.

Agar colonies: White, slow-growing, smooth, flat; edges entire, becoming undulate.

Milk: Becomes alkaline and clears.

Nitrites not produced from nitrates.

Indole test negative or feebly positive.

Hydrogen sulfide not produced (Okabe, Jour. Soc. Trop. Agr., 5, 1933, 166).

No growth in broth plus 4 per cent salt (Okabe, loc. cit.).

No gas from carbohydrates.

Temperature range, 1° C. to 35° C.

Source: Smith isolated the pathogen from blighted shoots of mulberry from Georgia. Also received cultures from Arkansas and the Pacific Coast.

Habitat: Pathogenic on mulberry, Morus.


119. Pseudomonas stizolobii (Wolf, 1920) Stapp, 1935. (Aplanobacter stizolobii Wolf, Phytopath., 10, 1920, 79; Stapp, Bot. Rev., 1, 1935, 405.)

sti.zo.lo'bi.i. Gr. stizo to prick, tattoo; Gr. dim. lobium a small lobe; M.L. neut.noun Stizolobium plant generic name; M.L. gen.noun stizolobii of Stizolobium.

Rods 0.6 to 0.7 by 1.0 to 1.6 microns.

Non-motile (Wolf, op. cit., 1920, 79). Motile with a short polar flagellum (McCulloch, Phytopath., 18, 1928, 460). Encapsulated. Gram-negative.

Gelatin: No liquefaction.

Agar colonies: Circular, smooth, white, raised and opaque. Margins entire to slightly undulate.

Broth: Slightly turbid throughout. No pellicle or ring.

Milk: Alkaline.

Nitrites not produced from nitrates.

Indole not produced.

No acid or gas in peptone broth plus sugars.

Starch not hydrolyzed.

Optimum temperature between 25° and 28° C.

Distinctive characters: Differs from Pseudomonas sojae (Pseudomonas glycinea) in the smaller size of cell, the absence of a pellicle and dense clouding of broth. The pathogen does not infect soybean.

Source: Isolated from the leaf spot of velvet bean.

Habitat: Pathogenic on velvet bean, Stizolobium deeringianium.


120. Pseudomonas viciae Uyeda, 1915. (Uyeda, in Takimoto, Jour. Plant Protect., Japan, 2, 1915, 845.)

vi'ci.ae. L. vicia vetch; M.L. fem.noun Vicia generic name of vetch; M.L. gen.nom. viciae of vetch.

Rods 0.5 to 0.8 by 1.2 to 2.0 microns. Motile with 2 to 4 polar flagella. Reported as Gram-positive; however, probably in error. No cultures are available for a retest of this character.

Green fluorescent pigment produced in culture.

Gelatin colonies: Pale white, glistening, finally turning brown. No liquefaction.

Milk: Coagulates and clears.

Nitrites not produced from nitrates.

Hydrogen sulfide not produced.

Aerobic, facultative.

Habitat: Pathogenic on the broad bean (Vicia faba), the turnip (Brassica rapa), the carrot (Dacus carota) and the sweet potato (Ipomoea batatas).


121. Pseudomonas alliicola (Burk holder, 1942) Starr and Burkholder, 1942.