Page:Bergey's manual of determinative bacteriology.djvu/204

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182
ORDER I. PSEUDOMONADALES

10. Pseudomonas pictorum Gray and Thornton, 1928. (Cent. f. Bakt., II Abt., 73, 1928, 89.) pic.to'rum. Named for the Picts, a Scot- tish tribe; M.L. neut.pl. gen. n. 'pictorum of the Picts. Rods 0.5 to 0.8 by 1.5 to 5.0 microns. Motile, usually with a single polar flagel- lum. Gram-negative. Gelatin colonies: Circular, greenish yellow, convex, smooth, glistening, entire. Gelatin stab: No liquefaction. Agar colonies: Circular, yellow, conve.x, smooth, glistening, entire. Agar slant: Filiform, yellow, convex, smooth, glistening, entire. Broth: Turbid. Nitrites produced from nitrates. Starch not hydrolyzed. Acid from glucose and maltose. Attacks phenol. Aerobic, facultative. Optimum temperature, 25°C. Source: One culture from soil. Habitat: Soil.

11. Pseudomonas arguta McBeth, 1916. (Soil Science, 1, 1916, 465.) ar.gu'ta. L. part. adj. argutus clear, bright. Rods 0.3 by 0.8 micron. Motile with one or two polar flagella. Gram-negative. Gelatin stab : Moderate, yellowish growth. No liquefaction in 30 daj^s. Agar colonies: Circular, slightly convex, soft, grayish white, granular, entire. Agar slant: Scant, grayish white growth. Potato agar slant: Moderate, yellowish, glistening growth. Broth: Turbid. Ammonia cellulose agar: Enzymatic zone 2 to 3 mm in 30 days. Filter paper broth: Paper is reduced to loose flocculent mass which disintegrates very readily on slight agitation. More rapid decomposition when the broth contains ammonium sulfate, potassium nitrate, pep- tone or casein as source of nitrogen. Litmus milk: Acid, not digested. Potato: No growth. Indole not produced. Nitrites produced from nitrates. Ammonia not produced. Acid from glucose, maltose, lactose and starch. No acid from glycerol, mannitol or sucrose. Aerobic, facultative. Optimum temperature, 20° C. Source: Isolated twice from California soils. Habitat: Soil.

12. Pseudomonas subcreta McBeth and Scales, 1913. (Bur. Plant Industry, U. S. Dept. Agr., Bull. 266, 1913, 37.) sub.cre'ta. L. pref. sub- somewhat; L. creta chalk; M.L. adj. subcretus somewhat chalky. Rods 0.3 by 1.4 microns. Motile with one to five polar flagella. Gram-negative. Gelatin stab: Filiform growth, no lique- faction. Cellulose agar: No surface growth. Moderate, generally faint yellow growth in medium, area of growth sunken. Agar slant: Glistening, smooth, moist, vitreous to faint yellow growth. Starch agar: Enzymatic zone 2 to 4 mm. Broth: No growth. Litmus milk: No growth. Potato: Growth scant, concave due to slight liquefaction, white to faint yellow. Bleached around growth. Indole not produced. Trace of nitrites produced from nitrates. Ammonia not produced. Acid from glucose, lactose, maltose, sucrose and starch. No acid from glycerol or mannitol. Aerobic, facultative. Optimum temperature, 20° C. Habitat: Soil.

13. Pseudomonas lacunogenes Gores- line, 1933. (Jour. Bact., £6, 1933, 447.) la.cu.no'ge.nes. L. lacuna a hollow; L. gicjno to produce; M.L. adj. lacunogenes pro- ducing hollows. Short rods, 0.2 to 0.3 by 1.0 to 1.2 microns, with pointed ends, occurring singly or in pairs. Motile with a single polar flagellum from 2 to 15 microns in length. Gram-nega- tive. Plain gelatin stab: No growth. Nutrient gelatin stab: Growth brownish yellow, half-way down stab, heavier at surface. No liquefaction.