Page:Bergey's manual of determinative bacteriology.djvu/225

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FAMILY IV. PSEUDOMONADACEAE
203

No growth in 6 per cent sodium chloride broth.

Source: Five strains were isolated from soil.

Habitat: Presumably soil.

2. Alginomonas terrestralginica (Waksman et al., 1934) Kåss et al., 1945. (Bacterium terrestralginicum Waksman, Carey and Allen, Jour. Bact., 28, 1934, 217; Kiss, Lid and Molland, Norske Videnskaps-Akad., Oslo, 1 Mat.-Naturv. Klasse, No. 11, 1945, 9.)

ter.res.tral.gi'ni.ca. L. noun terrestris land, earth; M.L. adj. alginicus pertaining to alginic acid from seaweed; M.L. adj. terrestralginicus land-alginic; presumably intended to mean an alginic bacterium from the soil.

Long rods, 1.0 to 1.5 by 1.5 to 2.5 microns, with somewhat rounded ends, usually occurring singly but also in pairs, occasionally in chains of shorter rods. Motile. Granular. Gram-negative.

Alginic acid plate: Colonies small, whitish in appearance with a slight metallic sheen.

Alginic acid liquid medium: Medium at first clouded. Later a pellicle is formed on the surface of the medium; it is soon broken up due to active gas formation. Reaction of medium becomes slightly alkaline.

Gelatin medium: Slow growth throughout stab, slow liquefaction at surface of medium at 18° C.

Agar liquefaction: None.

Glucose broth: Abundant turbidity; some sediment; no pellicle; slightly fluorescent.

Litmus milk: Acid; milk coagulated; only limited digestion of coagulum.

Potato: Abundant, pinkish, compact, dry growth on surface of plug, the rest of the plug becoming gray with a tendency to darkening.

Starch plate: Limited growth along streak; no diastase.

Aerobic to facultatively anaerobic.

Optimum temperature, 30° C.

Source: Isolated from New Jersey soil.

Habitat: Soil.


3. Alginomonas alginovora (Waksman et al., 1934) Kiss et al., 1945. (Bacterium alginovorum Waksman, Carey and Allen, Jour. Bact., 28, 1934, 215; K&ss, Lid and Molland, Norske Videnskaps-Akad., Oslo, I Mat.-Naturv. Klasse, No. 11, 1945, 9.)

al.gi.no'vo.ra. L. fem.n. alga seaweed; M.L. noun acidum alginicum alginic acid (derived from seaweed); L. v. voro to devour; M.L. adj. alginovorus alginic acid-destroying.

Rods, 0.75 to 1.2 by 1.5 to 2.0 microns, with rounded to almost elliptical ends, especially when single, occurring frequently in pairs and even in chains. Encapsulated. Actively motile. Gram-negative.

Alginic acid plate: Colony large, white in appearance, with coarse, granular center, entire margin. Clears up turbidity caused by alginic acid on the plate. No odor.

Alginic acid liquid medium: Heavy pellicle formation. Active production of an enzyme, alginase, which brings about the disappearance of alginic precipitate in seawater medium.

Salt-water medium: A slimy pellicle of a highly tenacious nature is produced, the whole medium later turning to a soft jelly.

Sea-water gelatin : Active and rapid liquefaction in two to six days at 18° C; highly turbid throughout the liquefied zone.

Agar liquefaction: Extensive softening of agar, no free liquid.

Sea-water glucose broth: Abundant, uniform turbidity with surface pellicle; some strains give heavier turbidity, and others heavier pellicle.

Litmus milk containing 3.5 per cent salt: No apparent growth.

Potato moistened with sea water: Moist, spreading, ivory-colored growth; heavy sediment in free liquid at the bottom.

Starch plate: Abundant, cream-colored, slimy growth; extensive diastase production.

Aerobic to microaerophilic. Optimum temperature, 20° C.

Source: Isolated from sea water, seabottom sediments and from the surface of algal growth in the sea.

Habitat: Very common in the sea.


4. Alginomonas fucicola (Waksman et al., 1934) Kiss et al., 1945. (Bacterium fucicola Waksman, Carey and Allen, Jour. Bact.,