Page:Bergey's manual of determinative bacteriology.djvu/257

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FAMILY VII. SPIRILLACEAE
235

Agar slant: Grayish white, slimy, entire growth. Broth: Turbid with flocculent sediment. Litmus milk: Unchanged. Potato: Not reported. Indole not produced. Blood serum peptonized. Starch not hydrolyzed. Acid from glucose, fructose, maltose, glycerol and sorbitol. Nitrites produced slowly from nitrates. Aerobic, facultative. Optimum temperature, 37° C. Source: Isolated from an abscess of the pectoral muscle of an African toad.

7. Vibrio leonardii Mdtalnikov and Chorine, 1928. (Ann. Inst. Past., Ji2, 1928, 1647.) le.o.nar'di.i. M.L. gen. noun leonardii of Leonard; named for A. G. Leonard. Curved rods with rounded ends, 0.5 to 1.0 by 2.0 to 3.0 microns. Motile by means of 1 to 3 polar flagella. Gram-negative. Gelatin stab: No liquefaction. Agar colonies: Small, transparent, circu- lar, having a characteristic odor. Broth: Turbid, with thin pellicle. Litmus milk: No coagulation, acid, with reduction of litmus. Potato: Slight, colorless growth. Coagulated blood serum not liquefied. Indole not produced. Hydrogen sulfide produced. Acid and gas from glucose, fructose, ga- lactose, lactose, sucrose and mannitol. No acid or gas from maltose or glycerol. Nitrites produced from nitrates. Aerobic, facultative. Optimum temperature, 30° C. Habitat: Highly pathogenic for insects such as Galleria mellonella L. (wax moth) and Pyrmista nubialis Hiibn. (European corn borer).

8. Vibrio marinopraesens ZoBell and Upham, 1944. (Bull. Scripps Inst, of Ocean- ography, Univ. Calif., 5, 1944, 256.) ma.ri .no.prae'sens. L. adj. marinus of the sea; L. part. adj. praesens present; M.L. adj. marinopraesens present in the sea. Curved rods, 0.4 to 0.5 by 1.2 to 2.4 mi- crons, occurring singly and in spiral chains. Polar staining. Motile by means of one or two polar flagella. Gram-negative. Note: All differential media except the fresh-water broth, litmus milk and potato were prepared with sea water. Gelatin colonies: Circular, 1 mm in di- ameter, dense center, brown discoloration of gelatin. Gelatin stab: Stratiform above, infundi- buliform below; complete liquefaction in 5 days; brown discoloration of gelatin. Agar colonies: Convex, circular, 0.5 mm in diameter, entire, translucent. Agar slant: Abundant, filiform, glisten- ing, butyrous growth with no pigment. Sea-water broth: Heavy turbidity; slight viscid sediment; surface ring. Fresh-water broth: No visible growth. Litmus milk: Completely decolorized. Potato: No visible growth. Indole not produced. Hydrogen sulfide rapidly produced. Acid but no gas from glucose and maltose. Glycerol, xylose, lactose, sucrose, mannitol and salicin not fermented. Starch is hydrolyzed. Non-lipolytic. Nitrites not produced from nitrates. Ammonia produced from peptone but not from urea. Casein not digested. Aerobic, facultative. Optimum temperature, between 20° and 25° C. Source: Isolated from sea water. Habitat: Common; probably widely dis- tributed.

9. Vibrio phytoplanktis ZoBell and Upham, 1944. (Bull. Scripps Inst, of Ocean- ography, Univ. Calif., 5, 1944, 261.) phy.to.plank'tis. Gr. neut.n. phylum plant; Gr. adj. plancius wandering; M.L. neut.n. plankton (plancium) plankton; M.L. neut.n. phytoplankton plant plankton; M.L. adj. ^phytoplanktis of the phytoplankton. Curved rods, 0.5 to 0.6 by 2.0 to 5.4 mi- crons, occurring mostly singly with some short spiral chains. Bipolar staining. Motile by means of a single polar flagcllum. Gram- negative. Note: All differential media except the