Gelatin colonies: Small, j^ellowish white. Gelatin stab: Liquefaction. Growth at the surface and along the stab. Agar: Abundant growth. Agar slant: Growth dull and wrinkled. Blood agar: Good growth and lumines- cence; beta hemolysis. Broth: Pellicle formed. Koser's citrate medium: Growth and lu- minescence. Milk: Growth. Potato: Luxuriant growth. Indole produced. Hydrogen sulfide not produced. Acid but no gas from glucose and sucrose. No acid or gas from lactose. Starch hydrolyzed. Gives a cholera-red reaction, i.e., pro- duces both indole and nitrites. Nitrites produced from nitrates. Optimum salt concentration, 0.9 per cent. Temperature relations: Optimum, 22° C.; growth at 37.5° C. Aerobic, facultative. Distinctive characters: Morphologically and culturally like Vibrio comma. Lumi- nescent. Pathogenic to guinea pigs and pigeons. Source: Originally isolated from the Elbe River. If Vibrio phosphorescens Jermoljewa (Cent. f. Bakt., I Abt., Orig., 100, 1926, 170) is accepted as identical with this spe- cies, then it has also been found in the in- testinal contents of three cholera patients, one gastroenteritis and one typhoid pa- tient; Jermoljewa (ibid., 171) also isolated his organism from the bile of a cadaver. Sonnenschein (op. cit., 12S, 1931, 92) reiso- lated this species from a fish taken from the Elbe River and found that it main- tained its ability to luminesce when grown in o.x bile. Habitat: Found in fresh water, in human feces and in bile. Probably widely distrib- uted.
15. Vibrio pierantonii (Zirpolo, 1918) Meissner, 1926. (Bacillus pierantonii Zir- polo. Boll. Soc. Nat. Napoli, 30, (1917) 1918, 206; Meissner, Cent. f. Bakt., II Abt., 67, 1926, 200.) pier.an.to'ni.i. M.L. gen. noun pierantonii of Pierantoni; named for Prof. U. Pieran- toni, an Italian scientist. Rods, 0.5 by 1.5 microns, with rounded ends. Rods curved and vibrio-shaped ac- cording to Meissner (ibid., 201). Motile by means of one to three polar flagella. Gram- negative. Gelatin colonies: Circular and irregularly lobulate. Gelatin stab: No liquefaction. Agar colonies: Circular, light green, smooth, entire. Glycerol agar slant: Slightly luminous streak. Broth: Turljid, with pellicle. Indole not produced. Acid from glucose and maltose. Some strains also attack lactose, sucrose and mannitol. Best growth in alkaline media. Aerobic, facultative. Optimum temperature, 37° C. Source: Isolated from the photogenic organ of the cephalopod Sepiola intermedia Naef.
16. Vibrio agarliquefaciens (Gray and Chalmers, 1924) Bergey et al., 1934. (Mi- crospira agar-liquefaciens (sic) Gray and Chalmers, Ann. Appl. Biol., 11, 1924, 325; Bergey et al., Manual, 4th ed., 1934, 119.) a.gar.li.que.fac'i.ens. Malay agar, a jelly from seaweeds; L. v. liquefacio to liquefy; M.L. part. adj. agarliquefaciens liquefying agar. Short, curved rods, usually c-shaped, with occasional s-shaped and coccoid forms. Cells 2.0 microns long by 0.5 to 0.7 micron broad; 3.0 to 5.0 microns long in division stages. Coccoid forms stained, 0.5 to 0.7 micron long. Motile by means of a single, polar flagellum. Gram stain not reported. Gelatin stab: Very slight surface growth after one month; the streak then shows a beaded line. No liquefaction. Agar colonies: Surface colonies appear as a whitish growth in a depression, sur- rounded by a white ring. The colony is later surrounded by a ring of liquid agar. Deep colonies show a clear area and may be irreg- ular, oval or angular. Agar slant: A deep groove is cut along
