Acid from glucose and sucrose. Occasion- ally from maltose and glycerol. Starch hydrolyzed. Nitrites produced from nitrates. Attacks phenol and naphthalene. Aerobic, facultative. Optimum temperature, between 30° and 35° C. Source: Fifteen cultures were isolated from soil . Habitat: Soil. 3. Achroniobacter thalassius ZoBell and Upham, 1944. (Bull. Scripps Inst, of Oceanography, Univ. of Calif., 5, 1944, 279.) tha.las'si.us. Gr. adj. thalassius of the sea. Rods, 0.6 to 0.7 by 0.8 to 2.3 microns, with some variation in shape, occurring singly, in pairs and in short chains; many cells lie side by side. Motile by means of peritrichous flagella. Gram-negative, but cell walls tend to retain stain. All media except the fresh-water broth, litmus milk and potato were prepared with sea water. Gelatin colonies: 1 mm in diameter, circular, white. Gelatin stab: Napiform liquefaction. Filiform growth along line of stab. Agar colonies: Punctiform, rough, trans- lucent, raised. Agar slant: Moderate, glistening, beaded, watery, butyrous growth with no pigment. Sea-water broth: No pellicle; slight turbidity; scant, powdery sediment. Fresh-water broth: Fair growth. Litmus milk: No visible change. Casein not digested. Potato: No visible growth. Indole not produced. Hydrogen sulfide not produced. Glucose, lactose, maltose, sucrose, xylose, mannitol, glycerol and salicin not utilized. Starch not hydrolyzed. Non-lipolytic. Of 19 amino acids tested, none was re- quired for growth; preformed growth fac- tors also were not required (Campbell and Williams, Food Research, 16, 1951a, 506). Ammonium chloride and the 19 amino acids which were tested may serve as sources of nitrogen; the amino acids may also be utilized as carbon sources (Campbell and Williams, loc. cit.). Nitrites produced from nitrates. Ammonia produced from peptone but not from urea. Trimethylamine not produced from tri- methylamine oxide, betaine, choline or acetyl choline (Campbell and Williams, Jour. Bact., 62, 1951b, 250). Inorganic sulfur may serve as a source of sulfur (Campbell and Williams, op. cit., 1951a, 506). Aerobic, facultative. Optimum temperature, between 20° and 25° C. Source: Isolated from marine bottom deposits. 4. Achroniobacter delicatiilus (Jor- dan, 1890) Bergey et al., 1923. (Bacillus delicatiilus Jordan, Report Mass. State Bd. of Health, 1890, 837; Bergey et al., Manual, 1st ed., 1923, 137.) de.li.ca'tu.lus. L. adj. delicatus dainty, delicate; M.L. dim. adj. delicatiilus some- what delicate. Original descriptions supplemented by Bergey {loc. cit.) from his private notes. Rods, 1.0 by 2.0 microns, occurring singly (Jordan). Motile by means of peritrichous flagella. Gram-negative (Bergey). Gelatin colonies: Whitish, homogeneous; radiate margin. Gelatin stab: Infundibuliform liquefac- tion. Agar slant: Whitish, glistening. Broth: Turbid; gray pellicle; sediment. Litmus milk: Acid. Potato: Thin, gray streak. Indole not produced (Bergey). Of 19 amino acids tested, none was re- quired for growth; preformed growth factors also were not required (Campbell and Williams, Food Research, 16, 1951a, 506). Ammonium chloride and the 19 amino acids which were tested may serve as sources of nitrogen; the amino acids may also be utilized as carbon sources (Campbell and Williams, loc. cit.). Nitrites produced from nitrates. Trimethylamine produced from tri- methylamine oxide, choline and acetyl
