Habitat: Presumably widely distributed in nature. 13. Flavobacteriuni harrisonii Bergey et al., 1923. (Variety No. 6, Harrison, Rev. gen. du Lait, 5, 1905, 129; Bacillus lactis harrisonii Conn, Esten and Stocking, Ann. Rept. Storrs Agr. Exp. Sta., 1906, 169; Bergey et al., Manual, 1st ed., 1923, 104.) har.ri.so'ni.i. M.L. gen. noun harrisonii of Harrison; named for Prof. F. C. Harrison, the Canadian bacteriologist who first isolated this species. Rods, 0.25 to 0.75 by 0.3 to 3.5 microns, occurring singly and occasionally in short chains. Motile by means of peritrichous flagella. Gram-negative. Gelatin colonies: Small, gray, glistening, lobular, citron-yellow, slimy. Gelatin stab: Villous growth in stab. Slow crateriform to napiform liquefaction. Agar slant: Growth luxuriant, viscous, spreading, becoming dirty to brownish citron-yellow. Broth: Turbid, with viscid ring and gelatinous sediment; sweetish odor; alka- line. Litmus milk: Colorless to gray and slimy, becoming yellow, alkaline. Potato: Luxuriant, yellow, spreading, slimy growth. Indole not produced. Glucose, lactose, maltose and sucrose broths turn alkaline with a disagreeable odor. Reaction of glycerol broth remains neutral. Aerobic, facultatively anaerobic. Optimum temperature, 25° C. Source: Isolated from slimy milk. Habitat: Unknown. 14. Flavobacteriuni diffusuni (Frank- land and Frankland, 1889) Bergey et al., 1923. (Bacillus diffusus G. and P. Frankland, Ztschr. f. Hyg., 6, 1889, 396; Bergey et al., Manual, 1st ed., 1923, 100.) dif.fu'sum. L. adj. diffustis spreading, diffuse. Original description supplemented by Harrison (Canadian Jour. Res., 1, 1929, 233) as indicated. Rods, 0.5 by 1.5 microns, occurring singly and in chains. Motile, possessing peritrich- ous flagella. Gram-negative (Harrison). Gelatin colonies: Thin, bluish green, spreading, later faint yellow. Gelatin stab: Thin, glistening, yellowish green surface growth. Slow, crateriform liquefaction. Agar slant: Thin, light j-ellow, glistening growth (Frankland and Frankland); capu- cine-buff (yellow-red-yellow) growth (Har- rison) . Broth: Turbid, with greenish yellow sedi- ment. Litmus milk: Unchanged (Harrison). Potato: Thin, smooth, greenish j^ellow, glistening growth. Indole not produced (Harrison). Slight acid from glucose. No acid from sucrose or lactose (Harrison). Nitrites produced from nitrates (Harri- son). Aerobic, facultatively anaerobic. Optimum temperature, between 25° and 30° C. Source: Originally found in soil. Found also by Tataroff (Die Dorpater Wasserbak- terien, Dorpat, 1891, 58) in fresh water and by Harrison from the skin of halibut from both the Atlantic and Pacific shores of Canada. Habitat: Soil, fresh and sea waters. 15. Flavobacteriuni rigense Bergej' et al., 1923. (Bacillus brunneus rigensis Baza- rewski. Cent. f. Bakt., II Abt., 15, 1905, 1; Bergey et al., Manual, 1st ed., 1923, 100.) ri.gen'se. M.L. adj. rigensis pertaining to Riga; named for Riga, the city where this species was isolated. Rods, 0.75 by 1.7 to 2.5 microns, occurring singly. Motile by means of peritrichous flagella. Gram-negative. Gelatin colonies: Circular, entire to undu- late, grayish white, homogeneous. Gelatin stab: Smooth, j'ellowish surface growth. Infundibuliform liquefaction. Brownish yellow sediment. Agar slant: Narrow, whitish streak, be- coming yellowish brown, spreading. Pig- ment is water- and alcohol -soluble but insoluble in ether. Broth: Turbid with pellicle and brownish sediment. Cells encapsulated.
