Page:Bergey's manual of determinative bacteriology.djvu/342

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320
ORDER IV. EUBACTERIALES

Gelatin stab: Rapid, stratiform liquefac- tion. Medium becomes brown. Agar colonies: Small, circular, smooth, yellow, amorphous, undulate margin. Agar slant: Moderate, flat, glistening, opaque, butyrous, yellow, with aromatic odor. Broth: Turbid with scant sediment. Aromatic odor, becoming cheesy. Litmus milk: Peptonized. Alkaline. Potato: Abundant, yellow, glistening growth becoming brown. Indole produced. Nitrites not produced from nitrates. Hydrogen sulfide produced. Slight acid but no gas from glucose, su- crose and glycerol. No acid from lactose. Starch is hydrolyzed. Blood serum is liquefied. Aerobic, facultatively anaerobic. Optimum temperature, 25° C. Source: Isolated from dairy wastes. Habitat: Unknown. 21. Flavobacterium niarinum Har- rison, 1929. (Canadian Jour, of Research, 1, 1929,234.) ma.ri'num. L. adj. marinus marine, of the sea. Rods, 0.8 by 1.2 to 1.3 microns, with rounded ends, occurring singly and in pairs. Motile by means of 4 to 5 peritrichous flagella. Encapsulated. Gram-variable; show blue granules in Gram-negative rods. Gelatin colonies: Circular, iridescent, whitish margin with pale yellow center. Liquefaction. Gelatin stab: Saccate to stratiform lique- faction. Agar colonies: Circular, pale yellow, smooth, convex, granular; reticulate edge. Agar slant: Amber-j^ellow, slightly raised, spreading, smooth, glistening, transparent growth. Ammonium phosphate agar: Scant growth. Broth: Turbid, sediment. Litmus milk: Alkaline. Digestion without coagulation. Clear serum. Potato: Abundant, amber-yellow, becom- ing dirty yellow, spreading, glistening growth. Indole not produced. Hydrogen sulfide not produced. Faint acidity from glucose; no action on lactose or sucrose. Nitrites not produced from nitrates. Trace of ammonia formed. Loeffler's blood serum not liquefied. Faint yellow, spreading growth. Aerobic, facultatively anaerobic. Optimum temperature, between 20° and 25° C. Source: Isolated from living halibut ob- tained at 30 to 50 fathoms. Pacific Ocean. Gibbons (Contrib. to Canadian Biol, and Fish., 8, 1934, 279) reports this species as occurring in the slime and feces of cod (Gadus callarias), halibut {Hippoglossus hippoglossus) and skate {Raja erinacea). Habitat: Skin and feces of fishes. 22. Flavobacterium lactis Bergey et al., 1923. {Bacillus aromaticus lactis Grimm, Cent. f. Bakt., II Abt., 8, 1902, 584; Bergey et al.. Manual, 1st ed., 1923, 108.) lac'tis. L. noun lac milk; L. gen. noun lactis of milk. Rods, 0.7 to 1.0 by 3.5 to 4.0 microns, oc- curring singly, in pairs and in chains. Motile by means of peritrichous flagella. Gram- negative. Gelatin colonies: Circular, light j-ellow, slimy, concentrically ringed, undulate. Gelatin stab: Slimy surface growth. No liquefaction. Agar slant: Slimy, j-ellowish, smooth, moist. Broth: Turbid, with abundant sediment. Litmus milk: Slightly acid. Potato: Growth thick, slimy, brownish, with yellowish margin. Indole not produced. Nitrites not produced from nitrates. Cultures have pleasant odor. Aerobic, facultatively anaerobic. Optimum temperature, 25° C. Source: Isolated from milk. Habitat: Unknown. 23. Flavobacterium tlevorans (Zimmer- mann, 1890) Bergey et al., 1923. {Bacillus devorans Zimmermann, Bakt. unserer Trink- u. Nutzwasser, Chemnitz, 1, 1890, 48; Bergey et al., Manual, Ist ed., 1923, 102.)