8. Agarbacteriuni bufo Angst, 1929. (Puget Sound Biol. Sta. Pub., 7, 1929, 53.) bu'fo. L. noun b^lfo the toad. Short rods, 0.6 by 0.8 micron, with rounded ends, occurring singly and in pairs. Not encapsulated. Non-motile. Gram- negative. All media contained a decoction made by boiling or autoclaving fronds of Iridaea, a seaweed, in sea water or a 3 per cent salt solution. Fish gelatin colonies: Circular, sunken, crateriform, granular, yellow; growth is slow. Fish gelatin slant: Scant, filiform, flat, glistening, smooth, translucent, white to yellowish, butj-rous growth. Fish gelatin stab : Stratiform liquefaction ; growth best at top. Agar colonies: Circular, concentrically ringed, sunken, entire, granular, yellow; growth is slow. Agar slant: Abundant, filiform, raised, glistening, contoured, opaque, j^ellow, mem- branous growth. Fish agar slant: Abundant, filiform, flat, glistening, smooth, opaque, yellow to orange, butyrous growth. Meat agar slant: Abundant, filiform, flat, glistening, contoured, opaque, yellow to orange, butyrous growth surrounded by a white halo. Broth: Moderately turbid; scant sedi- ment; granular, membranous pellicle. Plain milk unchanged; surface growth yellow. Potato: No growth. Indole not produced. Hydrogen sulfide not produced. Slight acid from mannitol. No acid from glucose, sucrose, lactose, xylose, rhamnose or arabinose. Starch not hydrolyzed. Agar digested; cellulose not attacked. Nitrites produced from nitrates. Aerobic. Methylene blue reduced. Temperature relations: Optimum, be- tween 25° and 28° C. Minimum, between 5° and 10° C. Maximum, less than 36° C. Source: Isolated from Odonthalia kam- tschatica. Habitat: Found on marine algae. 9. Agarbacteriuni polysiphoniae (Lundestad, 1928) Breed, comb. nov. {Bac- terium polysiphoniae Lundestad, Cent. f. Bakt., II Abt., 75, 1928, 331.) po.ly.si.pho'ni.ae. M.L. noun Polysi- phonia a genus of red algae; M.L. gen. noun polysiphoniae of Polysiphonia. Rods, 0.5 to 0.6 by 2.0 to 4.0 microns, with rounded ends, occurring singly. Non-motile. Gram-negative. Fish gelatin colonies: Circular, slightl}- glistening, bright .yellow, transparent, with dense center. Fish gelatin stab : Slight, yellowish growth on surface. Slow, saccate liquefaction. Seaweed agar colonies : Circular, flat, light yellow, with concentric rings and diffuse margin. Agar is disintegrated. Fish agar slant : Yellow, flat growth with undulate margin. Broth: Turbid; flocculent pellicle; 5'ellow- ish sediment. Indole not produced. Carbohydrates not attacked. Starch slightly hydrolyzed. Nitrites not produced from nitrates. Aerobic, facultatively anaerobic. Temperature relations: Optimum, 25° C. Minimum, between 5° and 10° C. Maximum, between 27° and 30° C. Source: Isolated from sea water from the Norwegian Coast. Habitat: Sea water. 10. Agarbacteriuni ceramicola (Lund- estad, 1928) Breed, comb. nov. (Bacterium ceramicola Lundestad, Cent. f. Bakt., II Abt., 75, 1928, 332.) ce.ram.i'co.la. M.L. noun Ceramium a genus of red algae; L. v. colo to dwell; M.L. fem.n. ceramicola the Ceramium dweller. Rods, 0.5 to 0.6 by 1.4 to 2.4 microns, with rounded ends, occurring singly and Ij'ing side by side. Non-motile. Gram-negative. Fish gelatin colonies: Circular, glistening, transparent, yellow. Fish gelatin stab: Slight, yellow surface growth. Crateriform liquefaction. Sea-water agar colonies: Circular, flat, transparent, glistening, light yellow; difi"use margin. Agar is disintegrated. Fish agar slant: Moderate, yellow, flat, entire, glistening, opaque growth.
