Serum gelatin: No liquefaction. Serum agar: Surface colonies small, trans- lucent, slightly raised, adherent to medium in 48 hours. Deep colonies lenticular, 2 mm in size in 48 hours. Colonies difficult to break up. No gas. Serum broth: Supernatant fluid clear with small, stellate colonies which tend to adhere to walls of the tube. No gas. Milk: Unchanged. Coagulated egg white and serum not liquefied. Indole not produced in serum peptone water. Hydrogen sulfide not produced. No acid or gas from carbohydrates. Anaerobic. Optimum temperature, 37° C. Pathogenic for guinea pigs, rabbits and mice. Source: Isolated from epidemic benign cervical adenitis of guinea pigs. Habitat: Infected guinea pigs so far as known. 17. Sphaerophorus glycolyticus Tar- dieux and Ernst, 1951. (Tardieu.x and Ernst, in Tardieux, Ann. Inst. Past., 80, 1951, 278.) gly.co.ly'ti.cus. Gr. adj. glycus sweet; Gr. adj. lyticus dissolving; M.L. adj. glyco- lyticus sugar-dissolving. Pleomorphic rods which, on initial cul- turing, occur as ellipsoidal forms, 2 to 3 microns in diameter, and as elongated forms with lateral or terminal, spheroidal swell- ings and metachromatic granules. With subsequent transfers, the short forms pre- dominate, especially in liquid media, and pleomorphism is retained in agar stabs. The ellipsoidal forms show bipolar staining. Non-motile. Gram-negative. Serum or ascitic fluid is required for growth. No gas, but abundant odor is produced. Gelatin: Slow liquefaction (sometimes only after 1 month). Agar stab: Colonies are punctiform or lenticular. Glucose broth: Abundant, homogeneous turbidity. In glucose broth, the end-products of fermentation are acetic, butyric and lactic acids, hydrogen sulfide, ammonia, acetone and traces of aldehydes and skatole. Amines and alcohols are not produced. Peptone serum broth: Very slight tur- bidity. Milk: Rapid coagulation and digestion. Coagulated proteins not attacked. Acid but no gas from glucose, fructose, galactose, sucrose, lactose, maltose and glycerol. Starch is not hydrolyzed. Nitrites not produced from nitrates. Neutral red is reduced, progressively re- coloring. Anaerobic. Survives for several minutes at 70° C. Neither to.xin nor hemolysin produced. Pathogenic for guinea pigs but not for rabbits. Source: Two strains were isolated from genital infections and a third from a war wound. Habitat: Human sources so far as known. 18. Sphaerophorus bullosus (Distaso, 1912) Breed, comb. nov. (Bacillus bullosus Distaso, Cent. f. Bakt., I Abt., Orig., 62, 1912, 443; Bacteroides bullosus Castellani and Chalmers, Man. Trop. Med., 3rd ed., 1919, 960; Spherocillus bullosis (sic) Pr^vot, Ann. Inst. Past., 60, 1938, 300 (type species of genus Sphaerocillus Prevot, ibid., 297).) bul.lo'sus. L. noun6MZZaaknob;M.L. adj. bullosus knobbed. Small rods with rounded ends; at times long, slender, bifurcating forms occur with an enlargement at one pole or in the center. Motile by means of peritrichous flagella. Show bipolar staining. Gram-negative. Gelatin: No liquefaction. Glucose agar colonies: Deep colonies very small with fimbriate margin. Gas is pro- duced. Glucose broth: Flocculent growth falling to bottom. Some gas produced. Litmus milk: Slight acidity. No coagula- tion. Indole not produced. Acid from glucose. No acid from lactose or sucrose. Anaerobic. Optimum temperature, 37° C. Source: Isolated from the intestinal tract. Habitat: Found in the intestinal tract.
