Source: Isolated from twenty cases of puerperal fever (Schottmiiller), from in- fected tonsils (Prevot) and from the female genital tract (Foubert and Douglas). Habitat: Female genital tract and tonsils so far as known. 5. Peptococcus prevotii (Foubert and Douglas, 1948) Douglas, comb. nov. {Micro- coccus prevotii Foubert and Douglas, Jour. Bact., 56, 1948,25.) pre.vo'ti.i. M.L. gen. noun prevotii of Prevot; named for A. Prevot, a French bac- teriologist. Spherical cells, 0.6 to 1.5 microns in di- ameter, occurring singly, in pairs, tetrads and irregular groups. Non-motile. Not en- capsulated. Gram-positive. Gelatin: No liquefaction. Agar colonies: Circular, 0.5 to 1.0 mm in diameter, smooth, entire, low convex, trans- lucent or opaque, gray to grayish white, butyrous. Growth in fluid medium: In peptone yeast extract broth, moderately heavy growth in 72 hours, coarsely granular; no odor; gas is produced. Growth of some strains enhanced slightly by glucose. Litmus milk: Litmus reduced. Indole not produced. Hydrogen sulfide production slight, if at all. All strains utilize small amounts of glu- cose. Some strains produce slight acidity in glucose, fructose, galactose, mannose, mal- tose and raffinose. Cell suspensions decom- pose serine, threonine and purines with the formation of CO2 , NH3 , H2 and unidenti- fied products. Glutamate, histidine and other amino acids not decomposed. Nitrites not produced from nitrates; ni- trites not reduced. Egg albumen, beef serum and casein not attacked. Coagulase-negative. Catalase-positive. Anaerobic. Optimum temperature, 37° C. Growth be- tween 25° and 37° C. Optimum pH, between 6.5 and 8.5. Non-hemolytic. Distinctive characters : Distinguished from Peptococcus aerogenes by its failure to produce indole, to reduce nitrates and to ferment glutamate and histidine. Source: Isolated from the female genital tract; also from tonsils, from a bottle of plasma and from skin. Habitat: From human sources so far as known. 6. Peptococcus grigoroffii (Prevot, 1933) Douglas, comb. nov. (Micrococcus A, Grigoroff, These de Geneve, 1905; Micro- coccus grigoroffi, (sic) Prevot, Ann. Sci. Nat., Ser. Bot. et Zool., 15, 1933, 219.) gri.go.rof'li.i. M.L. gen. noun grigoroffii of Grigoroff; named for S. Grigoroff, the bacteriologist who first isolated this or- ganism. Small spheres, averaging 0.7 micron in diameter, occurring singly or in irregular masses. Gram-positive. Gelatin: Colonies appear in four days. No liquefaction. Deep agar colonies: After three days, round, lenticular, yellowish. Glucose broth: Turbid after two days with whitish sediment. Neither gas nor fetid odor produced. The medium is acidi- fied. Milk: Good growth; acid; coagulation. Acid from glucose, fructose, maltose, lac- tose and sorbitol. Anaerobic. Optimum temperature, 37° C. One strain is slightly pathogenic. Distinctive characters: This is the onl}- anaerobic coccus growing in irregular masses that coagulates milk. Lactose is fer- mented. Source: Five strains were isolated from the appendix by Grigoroff; one strain was isolated from an appendix by Prevot. Habitat: Human digestive tract. Not common. 7. Peptococcus constellatus (Prevot, 1924) Douglas, comb. nov. {Diplococcus con- stellatus Prevot, Compt. rend. Soc. Biol., Paris, 5/, 1924,426.) con.stel.la'tus. L. adj. constellatus stud- ded with stars. Description taken in part from Prevot
