Neutral red glucose broth: Becomes pink, but no further change. Strictly anaerobic. Temperature relations: Optimum, 37° C. No growth at 28° C. Optimum pH, between 7.0 and 8.0. Non-pathogenic. Distinctive characters: Colonies grow in narrow zone 1 cm below the surface of an agar stab; gas produced from peptones. Source: Isolated from bronchial mucus and from elsewhere in the respiratory sys- tem; also from dental and tonsillar}^ focal infections. Habitat: Found in the human buccal cavity and probably also in other warm- blooded animals. 4. Veillonella reniformis (Cottet, 1900) Pelczar, comb. nov. (Diplococcus reniformis Cottet, Compt. rend. Soc. Biol., 52, 1900, 421; Micrococcus reniformis Oliver and Wherry, Jour. Inf. Dis., 28, 1921, 341; Neisseria reniformis Prevot, Ann. Sci. Nat., Ser. Bot., 15, 1933, 102.) re. ni. for 'mis. L. noun ren kidney; L. noun forma shape; M.L. adj. reniformis kidney-shaped. Spheres, 0.8 to 1.0 micron in diameter, bean-shaped, occurring in pairs. Gram- negative. Gelatin: No liquefaction. Agar deep colonies: Appear in 24 to 48 hours; at first punctiform, then lenticular; small, 0.3 to 0.5 mm in diameter. No gas produced. Agar slant: Minute, bluish white, dew- drop colonies. Broth: Turbid in 24 hours; flocculent precipitate rapidly formed, clearing the medium. No gas produced, but a rancid odor is present. Peptone water: Very meager growth. Milk: Unchanged. Indole produced in trace amounts. Coagulated proteins: No digestion. Acid from glucose in slight amounts by only one strain. Strictly anaerobic. Temperature relations: Optimum, 37° C. No growth at 22° C. Optimum pH, 7.0. Limits of pH, 6.0 to 8.0. Pathogenic. Distinctive character: Odor of rancid butter. Source: Isolated in several cases from suppurations of the urogenital system. Habitat: Presumably found in bodies of warm-blooded animals. 5. Veillonella orbiculus (Tissier, 1908) Pelczar, comb. nov. {Diplococcus orbiculus Tissier, Ann. Inst. Past., 22, 1908, 204; Neisseria orbiculata (sic) Pr6vot, Ann. Sci. Nat.,S6r. Bot., ./J, 1933,109.) or.bi'cu.lus. L. mas. dim. n. orbiculus small disc. Spheres, 1.5 to 2.0 microns in diameter, occurring in pairs. Gram-negative. Gelatin: No growth at 22° C. Agar deep colonies: After 36 to 48 hours, large, lenticular, very regular, whitish, al- most transparent. Gas not produced. Broth: Turbid; sediment. Milk: No coagulation. Egg white: Not attacked. Proteoses attacked without the produc- tion of indole. Acid from glucose and feebly from lac- tose. No acid from sucrose. Strictly anaerobic. Temperature relations: Optimum, 37° C. No growth at 22° C. Non-pathogenic. Distinctive characters: Large size; no gas production. Source: Isolated from the feces of young children. Habitat: Found in the intestinal canal. Not common. 6. Veillonella vulvovaginitidis (Reynes, 1947) Pelczar, comb. nov. (Neisseria vulvo- vaginitis (sic) Reynes, Ann. Inst. Past., 73, 1947, 601.) vul.vo.va.gi.ni'ti.dis L. fem.n. vulva a covering, vulva; L. fem.n. vagina vagina; Gr. suffix -itis inflammation of; M.L. noun vulvovaginitidis vulvovaginitis. Spheres, about 0.8 to 1.0 micron in di- ameter, occurring in pairs or in very short chains, rarely singly, often in masses of 5 to 10 cells. Not encapsulated. Non-motile. Gram-negative. Gelatin: Liquefaction.
