non-motile, non-gelatin-liquefying species which possesses the characteristic diph- theroid morphology and which does not ferment sugars and related compounds; furthermore, it is less actively lipolytic than is the species described by Huss (op. cit., 1908, 474). Huss' organism appears to occur less frequently than that described by Evans. Source: Isolated from the udder of a cow giving abnormal milk. Habitat: Found in milk and in dair}^ products. 21. Brevibacterium acetylicum (Le- vine and Soppeland, 1926) Breed, comb. nov. (Flavobacterium acetylicxim Levine and Sop- peland, Bull. 77, Engineering Exp. Sta., Iowa State Agr. College, 1926, 46.) a.ce.ty'li.cum. L. neut.n. acetum vine- gar; M.L. neut.n. acetylum the organic radical acetyl; M.L. adj. acetylicus pertain- ing to acetyl. Rods, 0.9 by 1.1. microns, with rounded ends, occurring singly and in pairs. Motile (Levine and Soppeland). Peritrichous as determined by O. B. Weeks, January, 1955, from A.T.C.C. cultures 953 and 954. Gram- positive. Gelatin stab: Stratiform liquefaction. Agar colonies: Irregular in form, yellow, smooth, flat, amorphous, entire. Agar slant: Growth abundant, echinulate, flat, peach-yellow, smooth, butyrous. Broth: Ring growth on surface. Turbid with a scant sediment. Litmus milk: Slight acidity with a granu- lar curd; peptonized; litmus reduced. Potato: Moderate, orange growth. Indole not produced. Acid from glucose. Starch is hydrolyzed. Acetylmethylcarbinol is produced. Nitrites not produced from nitrates. Blood serum: Liquefaction. Aerobic, facultatively anaerobic. Optimum temperature, 22° C. Source: Isolated from skimmed milk. Habitat: Unknown. 22. Brevibacterium sulfureum (Bergey et al., 1923) Breed, comb. nov. {Bactermm punctans suljureuvi Zettnow, Cent. f. Bakt., I Abt., Orig., 77, 1916, 222; Flavobacterium sulfureum Bergey et al., Manual, 1st ed., 1923, 103.) sul.fu're.um. L. adj. sulfureus of sulfur, from sulfur-colored growth. Rods, 0.5 to 0.7 by 0.7 to 1.5 microns. Motile by means of peritrichous flagella. Gram-positive. Gelatin colonies: Very small, barely visible, becoming brownish yellow, granu- lar. Gelatin stab: Spreading growth on the surface only ; later, crateriform liquefaction. Agar slant: Sulfur-yellow growth. Broth: Turbid. Litmus milk: Alkaline; peptonized; yel- low. Potato: Sulfur-yellow streak. Indole not produced. No acid from glucose. Nitrites not produced from nitrates. Blood serum: Sulfur-yellow growth. Par- tial liquefaction. Aerobic, facultatively anaerobic. Optimum temperature, 25° C. Source: Isolated from air. Habitat : Unknown. 23. Brevibacterium helvolum (Zimmer- mann, 1890) Lochhead, 1955. {Bacillus hel- volus Zimmermann, Die Bakt. unserer Trink- und Nutzwasser, Chemnitz, 1, 1890, 52; Bacterium helvolum Lehmann and Neu- mann, Bakt. Diag., 1 Aufl., 3, 1896, 254; Corynebacterium helvolum Kisskalt and Berend, Cent. f. Bakt., I Abt., Orig., 81, 1918, 446; not Corynebacterium helvolum Jensen, Proc. Linn. Soc. New So. Wales, 59, 1934, 37; Flavobacterium helvolum Bergey et al., Manual, 1st ed., 1923, 114; Lochhead, Internat. Bull, of Bact. Nomen. andTa.xon., 5, 1955, 115.) hel'vo.lum. L. adj. helvolus pale yellow. Description given below is taken directly from the original description by Zimmer- mann {op. cit., 1890, 52) except as indicated. Lehmann and Neumann add certain charac- ters to the original description from a study of a culture from air that agreed with Zim- mermann 's culture. The description in Migula (Syst. d. Bakt., 2, 1900, 324) is ac- curately paraphrased from Zimmermann 's original description.
