Sodium hippurate not hydrolyzed. Source: Normal human throat. Not con- sidered to be pathogenic. Habitat: Human throat. 4. Streptococcus sp. Fry. (Group L, Hare and Fry, Vet. Rec, 1, 1938, 1537.) Addi- tional information from Laughton, Jour. Path. Bact., 60, 1948, 471. Spherical or ovoid cells in long chains. Gram-positive. Serology: Established as group L. Action on blood: Surface and submerged colonies are beta hemolytic. Most strains do not produce a demonstrable soluble hemolysin. Colony forms: Glossy, matt and inter- mediate types observed on blood agar. Final pH in glucose broth, between 4.7 and 5.2. Acid produced from maltose, lactose, sucrose, trehalose and salicin. May or may not ferment glycerol and sorbitol. Sodium hippurate not hydrolyzed. Source: Isolated from miscellaneous in- fections of the dog. Habitat : Probably the throat and genital tract of the dog. 5. Streptococcus sp. Fry. (Group M, Hare and Fry, Vet. Rec, 1, 1938, 1537.) Addi- tional information from Laughton, Jour. Path. Bact., 60, 1948, 471. Spherical or ovoid cells in long chains. Gram-positive. Serology: Established as group M. At least two serological types are known. Action on blood: Surface and submerged colonies are beta hemolytic. A weak, slow acting soluble hemolysin produced by some strains; other strains appear to be negative. Colony forms: Glossy, matt and inter- mediate types observed on blood agar. Final pH in glucose broth, between 4.6 and 6.9. Acid produced from maltose, lactose, sucrose and usually from trehalose. May or may not ferment glycerol and salicin. Sor- bitol not fermented. Sodium hippurate not hydrolyzed. Source: Isolated from the urethra, the vagina and the tonsillar area of dogs. Prob- ably not associated with any canine dis- ease. Habitat: Probably the dog. 6. Streptococcus sp. Boissard and Wor- mald. (Hitherto undescribed serological group O, Boissard and Wormald, Jour. Path. Bact., 62, 1950, 37.) Spherical or ovoid cells occurring in very long chains in broth culture. Serology: Belongs to a new serological group for which "group O" is proposed. Action on blood: Hemolysis on blood agar ranges from frank green to a moderately wide zone of beta hemolysis similar to that produced hy Streptococcus pyogenes. Growth under anaerobic conditions reduces or com- pletely inhibits the beta hemolysis. No soluble hemolysin detected. Colonj^ forms: Surface colonies on blood agar 0.4 to 0.8 mm in diameter after 18 hours at 37° C. with flattened margin and raised center. The margin is radically striated and has a beaded or pleated edge. Colony has rubbery and coherent consistency. Not fibrinolytic. Temperature relations: Does not survive 60° C. for 30 minutes. Tolerance tests: May or may not grow on blood agar containing 10 per cent bile. No growth on 40 per cent bile blood agar. Final pH in glucose broth, between 4.5 and 5.1. Acid produced from glucose and lactose. May or maj^ not ferment trehalose and sali- cin. No acid from mannitol or sorbitol. Sodium hippurate not hj'drolyzed. Not pathogenic for mice. Source: Nasopharynx of normal humans; occasionally from throats of individuals suffering from tonsilitis. Habitat: Probably the human naso- pharynx. 7. Streptococcus MG. Mirick, Thomas, Curnen and Horsfall. (Jour. Exp. Med., 80, 1944, .391, 407 and 431.) Spherical or ovoid cells occurring in pairs or short chains. Gram-positive. Serology: No group-specific antigen has been demonstrated. All strains belong to one serological type due to the presence of a type-specific capsular antigen shown to be
