and traces of lactic acid and acetylmethyl- carbinol. Neutral red reduced. Nitrites not produced from nitrates. Anaerobic. Optimum temperature, between 30° and 37° C. Optimum pH, between 6.5 and 8.2. Non-pathogenic for guinea pigs, rabbits and mice. Comments: A hemolj'tic variety of this organism has been reported by Beeuwkes and Aladame (Ann. Inst. Past., 75, 1948, 390). Source: Isolated from a subacute case of pulmonary gangrene. Habitat : Found in the natural cavities of man, especially the respiratory. 5. Peptostreplococcus lanceolatu.s (Prevot, 1933) Smith, comb. nov. {Coccus lanceolatus anaerobhis Tissier, Compt. rend. Soc. Biol., Paris, 94, 1926, 447; Streptococcals lanceolatus Prevot, Ann. Sci. Nat., Ser. Hot., 15, 1933, 173 and 193; not Streptococcus lanceolatus Gamaleia, Ann. Inst. Past., 2, 1888, 440.) lan.ce.o.la'tus. L. adj. lanceolatus lancet- shaped. Large, ovoid cells, 1.2 to 1.4 microns in diameter, with pointed ends, occurring in short chains in culture and in pairs in ex- udates. Non-motile. Gram-positive. Gelatin: No liquefaction. Deep agar colonies: Very large, lenticular; abundant gas produced which breaks up the medium. Peptone broth: Uniform turbidity; gran- ular, viscous sediment. Peptone broth: Good growth; gas pro- duced. Milk: No change. Coagulated protein not attacked. Hydrolyzed albumen reduced to CO3 , (NH4)2C03 and NH,,. Acid from glucose, sucrose and starch. No acid from lactose. Butyric, valerianic and acetic acids are produced, in the pro- portions 2:1: trace, from glucose and su- crose. Anaerobic. Optimum temperature, 37° C. Non-pathogenic for laboratory animals. Distinctive characters: Proteolytic and saccharolytic; produces ammonia from hydrolyzed proteins; butyric, valerianic and acetic acids are produced from certain carbohydrates. No hydrogen sulfide is pro- duced. Source: Isolated from human feces in a case of diarrhoea. Habitat: Found in putrefying materials. 6. Peptostreptococcus micros (Prevot, 1933) Smith, comb. nov. {Streptococcus an- aerobius micros Lewkowicz, Arch. Med. Exp., IS, 1901, 645; Streptococcus micros Prevot, Ann. Sci. Nat., Ser. Bot., 15, 1933, 193.) mi'cros. Gr. adj. micrus small. Very small spheres, 0.2 to 0.4 micron in diameter, occurring in long chains or in pairs. Non-motile. Gram-positive. Gelatin: Poor growth. No liquefaction. Semi-solid agar (Veillon) : Slow growth; colonies at first punctiform, becoming len- ticular and later forming processes into the medium. Average size, 0.5 to 1.0 mm in di- ameter; some reach 2 to 3 mm, growing 2 or 3 cm below the surface. Blood agar: No hemolysis. No hemopep- tolysis. Martin broth: Sliglit, particulate tur- bidity which slowly settles. Meat and liver broth: Rapid growth; abundant sediment. Peptone broth: Powdery sediment; me- dium not acidified; no indole is produced. Milk: Grows with difficulty; no acid; no coagulation. Coagulated protein not attacked. Acid from glucose, fructose, galactose, sucrose and maltose. Fermentation prod- ucts include propionic, formic and lactic acids (Pr(5vot, Man. d. Classif. et d. De- term, d. Bact. Anaerobies, 2" ed., 1948, 59). Neutral red broth is changed to fluores- cent yellow. Anaerobic. Optimum temperature, between 36° and 38° C. No growth at 22° C. Killed in 15 min- utes at 60° C. Optimum pH, about 7.0. Non-pathogenic for mice. No toxin and no hemolysin. Distinctive characters: Neither gas nor fetid odor produced; small size.
