Page:Bergey's manual of determinative bacteriology.djvu/580

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558
ORDER IV. EUBACTERIALES

Neutral red reduced. Anaerobic. Optimum temperature, 37° C. Optimum pH, between 6.5 and 7.8. Acid- tolerant but not acidophilic. Not pathogenic for guinea pigs or mice. Source: Isolated from a peptic digest of meat in an acid medium. Habitat: Unknown. 14. Eiibacterium aerofaciens (Eggerth, 1935) Prevot, 1938. (Bacteroides aerofaciens Eggerth, Jour. Bact., 30, 1935, 282; Prevot, Ann. Inst. Past., 60, 1938, 295.) a.e.ro.fa'ci.ens. Gr. mas.n. aer air, gas; L. V. facio to make, produce; M.L. part. adj. aerofaciens gas-producing. Ovoid rods, 0.4 to 2.0 by 2.0 to 3.0 microns, with rounded or pointed ends, occurring in chains of two to ten elements. Non-motile. Gram-positive. Gas produced in culture media. Gelatin: No liquefaction. Deep agar colonies : 1 to 2 mm in diameter, coherent. Glucose broth: Slightly turbid. Milk: Acidified; not coagulated. Coagulated proteins not attacked. Indole not produced. Hydrogen sulfide not produced. Acid and gas from glucose, maltose, fruc- tose, galactose, sucrose and lactose. Nitrites not produced from nitrates. Anaerobic. Pathogenic for mice. Source: Isolated from human feces. Habitat: Found in the human intestine. Rather uncommon. 15. Eubacteriiim parvum Prevot, 1938. (Coccobacillus anaerobicus parvus Chouke- vitch, Ann. Inst. Past., 25, 1911, 256; Pre- vot, Ann. Inst. Past., 60, 1938, 295.) par'vum. L. adj. parvus small. Small, ovoid rods, 0.5 by 1.0 to 1.5 mi- crons, occurring singly or in pairs; filamen- tous forms may occur. Non-motile. Gram- positive. Gas not produced in culture media. Gelatin: No liquefaction. Deep agar colonies: Small, lenticular. Glucose broth: Abundant turbidity and sediment. Milk: Coagulated in 20 to 25 days; some strains coagulate more rapidly ; no digestion. Coagulated proteins not attacked. Acid from glucose, fructose, maltose, galactose and lactose. Ammonia, aldehydes, alcohols, ketones, acetylmethylcarbinol and formic, butyric and lactic acids are produced. Nitrites not produced from nitrates. Neutral red and phenosafranin reduced. Anaerobic. Optimum temperature, 37° C. Not pathogenic for laboratory animals. Source: Isolated from the large intestine of a horse; also from a case of acute appendi- citis. Habitat: Found in the intestines of foals and of man. Uncommon. 16. Eubacterium crispatum Brygoo and Aladame, 1953. (Ann. Inst. Past., 8^, 1953, 640.) cris.pa'tum. L. part. adj. crispatus curled, crisped. Straight or slightly curved rods, 1.0 by 3.0 microns, occurring sometimes singly but usually in rather long chains having a twisted appearance and numerous loops; the length of each chain varies from 20 to 40 microns. Non-motile. Gram-positive. Gas not produced in culture media. Gelatin: No liquefaction. Deep agar colonies: Punctiform. Glucose broth: Abundant, uniform tur- bidity. Peptone broth: Slightly turbid. Milk: Slowly coagulated; partially di- gested. Coagulated proteins not attacked. Indole not produced. Hydrogen sulfide not produced. Acid from glucose, fructose, maltose, sucrose, lactose, galactose and starch. No acid from glycerol. Acetic acid and traces of lactic acid, but not amines, aldehydes, ketones or acetone, are produced. Nitrites not produced from nitrates. Sulfites not produced from sulfates. Neutral red reduced. Safranin and pheno- safranin not reduced. Anaerobic. Not pathogenic for guinea pigs or mice.