Page:Bergey's manual of determinative bacteriology.djvu/743

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FAMILY II. ACTINOMYCETACEAE
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Serum agar: Minute, round, colorless colonies with pinkish tinge in confluent, raised patch. Inspissated serum: Small, round, pale pink colonies; umbilicated and raised up. Broth: Liberal growth, white flocculent colonies; later pink surface colonies. Sj^nthetic sucrose solution: Colorless, flocculent sediment; thin, colorless pellicle. Milk: Surface growth; white aerial my- celium; solid coagulum; later partly pep- tonized with pink aerial mj'^celium. Litmus milk: Pink surface growth; aerial mj'celium; milky opaque after 40 days. Carrot plug: Small, irregularly round, raised colonies, colorless, covered with stiff aerial spikes; later buff-colored, convo- luted and ribbed growth with small patches of white, aerial mycelium; aerial mycelium pink in two months. Source: Isolated from a fatal case of lung disease and pericarditis in man. Habitat: Human infections so far as known. 7. Nocardia caprae (Silberschmidt, 1899) Waksman and Henrici, 1948. (Streptothrix caprae Silberschmidt, Ann. Inst. Past., 13, 1899, 841 ; Waksman and Henrici, in Manual, 6th ed., 1948, 899.) cap'rae. L. noun capra a she-goat; L. gen. noun caprae of a she-goat. Description taken from Erikson (Med. Res. Council Spec. Rept. Ser. 203, 1935, 26). Initial cells only slightly enlarged; early development of aerial hyphae while sub- stratum threads are still short; frequent slipping of branches; aerial mycelium abun- dant on all media with tendency to form co- herent spikes; mycelium not very polymor- phous, but occasional, thicker segments appear. Slightly acid-fast. Gelatin: Extensive dull growth with small, raised patches of pink, aerial myce- lium; later ribbon-like, depressed. No lique- faction. Glucose agar: Irregular, bright pink growth tending to be heaped up; later abun- dant masses frosted over with thin, white, aerial mycelium. Glycerol agar: Abundant growth; small round pink colonies partly covered with white aerial mycelium. Potato agar: Extensive, thin growth, pink in raised patches, covered by white, aerial mycelium; later aerial mycelium also be- comes pink. Starch agar: Minute, colorless colonies covered by white, aerial mycelium. Blood agar: Minute, round, colorless colonies aggregated in broad, pink zones; paler aerial mycelium. No hemolysis. Dorset's egg medium: Few, colorless colonies, some pink; white aerial mycelium; later, growth becoming dull pink, irregular, with scant white aerial mj^celium. Ca-agar: Minute, colorless colonies; white aerial mycelium; later a pinkish tinge. Serum agar: Small, round, pink colonies frosted over with thin, white, aerial my- celium. Inspissated serum: No growth. Broth: Superficial pellicle composed of pink colonies with white aerial mycelium; moderate, flocculent sediment. Glucose broth: Small sediment of fine flocculi; later pellicle composed of small pink colonies; superficial skin entire and salmon-colored in 16 days. Synthetic glycerol solution: Round, pink, disc-like colonies on surface and tenuous, white, wispy growth in suspension and sedi- ment; after 20 days, surface colonies bearing white aerial mycelium extending 2 cm up tube. Synthetic sucrose solution: Minute, white colonies in suspension and sediment in 3 days; thin, dust-like pellicle in 10 days; some surface colonies with white aerial mycelium in 17 days. Milk: Red surface skin; solid coagulum. Litmus milk: Red surface growth; no change in liquid; after 4 weeks, liquid de- colorized, opaque. Potato plug: Abundant growth; small colonies, mostly confluent, entirely covered with pale pink aerial mycelium; growth be- comes membranous, considerably buckled; later superficial colonies with pink aerial mycelium on liquid at base of tube; bottom growth of round white colonies. Starch not hydrolyzed. Source: Isolated from lesions in goats.