Page:Bergey's manual of determinative bacteriology.djvu/754

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ORDER V. ACTINOMYCETALES

small, round, pinkish, discrete colonies at margin. Glucose agar: Abundant, pale pink growth; small conical colonies, piled up, convoluted. Glycerol agar: Extensive, granular, ir- regular, thin, pinkish growth; after 40 days, a few discrete colonies with de- pressed margins, center piled up, pink. Serum agar: Smooth, cream, umbilicated colonies with submerged growth extending into medium in scallops 5 to 8 mm deep; a pale pink mass in 2 weeks. Potato agar: Small, round, colorless colonies covered with white aerial my- celium; after 2 weeks colonies dull pink; submerged margins; few aerial spikes; moderate aerial mycelium at top of slant. Broth: Flakes, later innumerable, minute colonies, some adhering to wall just above liquid level. Synthetic sucrose solution: Delicate, round, white colonies; later abundant minute colonies in suspension; thick cream pellicle on surface and pink grains in sedi- ment. Milk: Heavy, convoluted, bright yellow surface pellicle; no coagulation. Litmus milk: Yellow surface growth; milky sediment; liquid unchanged. Carrot plug: Small, round, smooth, cream-colored, elevated colonies in 10 days; sparse, stiff, colorless aerial spikes in 16 days; abundantly piled up, convoluted, ochreous growth in 25 days. Source: Isolated from the hock joint of a foal. Habitat: Unknown. 29. Nocardia cuniculi Snijders, 1924. (Geneesk. Tijdsch. Ned. Ind., 6^, 1924, 47 and 75.) cu.ni'cu.li. L. noun cuniculus a rabbit; L. gen. noun cuniculi of a rabbit. Description taken from Erikson (Med. Res. Council Spec. Rept. Ser. 203, 1935, 31). Large, swollen cells giving rise to ramify- ing filaments or to small chains of short, thick segments which branch out into more regular hyphae; sometimes the ir- regular elements are beset with spiny proc- esses before giving rise to typical long branching filaments; later the picture be- comes more monomorphous, and short, straight aerial hyphae are borne which pres- ently exhibit irregular segmentation. Gelatin: Few flakes. No liquefaction. Agar: Small, round, elevated, cream - colored colonies, umbilicated and radially wrinkled. Glucose agar: Minute, colorless colonies; becoming dull pink, partly confluent and piled up; few stiff pink aerial spikes. Glycerol agar: Small, round, elevated, cream-colored colonies; margins depressed; becoming smooth, discrete, yellowish. Dorset's egg medium: Scant, pinkish, smeary growth. Serum agar: Small, raised, cream-colored colonies, becoming confluent and piled up. Inspissated serum: Thick, colorless, ribbed membrane; no liquefaction. Broth: Small and larger cream-colored, scale-like surface colonies; abundant, flocculent bottom growth. Synthetic sucrose solution: Thin surface pellicle; small colorless flakes; minute par- ticles at bottom; scant growth. Milk: Heavy yellow growth attached to walls; solid coagulum in 1 month. Litmus milk: Yellow surface growth; liquid unchanged. Potato plug: Coral -pink, dry, granular growth covered to a considerable extent with white aerial mycelium, piled up in center, discrete colonies at margin, pink surface pellicle on liquid and colorless colonies at base. Source: Isolated from infected rabbits. Habitat: Unknown. 30. Nocardia rangoonensis (Erikson, 1935) Waksman and Henrici, 1948. (Actino- myces rangoon Erikson, Med. Res. Council Spec. Rept. Ser. 203, 1935, 37; Waksman and Henrici, in Manual, 6th ed., 1948, 911.) ran. goo. nen 'sis. M.L. adj. rangoonensis pertaining to Rangoon; named for Rangoon, Burma. Swollen, round initial cells, giving rise to branching hyphae which segment and present slipping and angular arrangement; few short straight aerial hyphae, which later develop into a profusely branching, long waving aerial mycelium. Non-acid-fast.