irregularly wrinkled, with small discrete colonies; clear hemolytic zone. Broth: Sediment of flocculi; some round and fan-shaped colonies. Synthetic sucrose solution: Very delicate, white flocculi. Milk: Coagulated; partly peptonized. Potato plug : No growth. Starch not hydrolyzed. Tyrosine agar: Negative reaction. Source : Isolated from the spleen in a case of acholuric jaundice. Injected into a monkey from which it was then reisolated. Habitat: Found in human infections so far as known. 36. Nocardia fructifera (Krassilnikov, 1941) Waksman, 1953. (Proactinomyces fruc- tiferi (sic) Krassilnikov, Guide to the Acti- nomycetes, Izd. Akad. Nauk, U.S.S.R., Moskau, 1941, 78; Waksman, in Waksman and Lechevalier, Actinomycetes and Their Antibiotics, Baltimore, 1953, 155.) fruc.ti'fe.ra. L. part. adj. fructifenis fruit-bearing. Mycelium septate, hyphae breaking up into rods and in some cultures into cocci. Aerial mycelium well developed, whitish to rose-colored. Sporophores long. Straight or weakly wavy, but not spiral-shaped. Oidiospores cylindrical, elongated, 0.7 by 1.5 microns. Not acid-fast. Gram-positive. Colonies not compact, mostly dough-like in consistency, smooth or rough. Gelatin: Slow liquefaction. Agar: Aerial mycelium weakly developed or entirely absent. Synthetic agar: Rose-colored to bright red and even red-orange growth. Pigment not soluble in medium. Milk: Coagulated and weakly peptonized. Sucrose is inverted. Starch weakly hydrolyzed. Cellulose: Poor growth. Fats: Good growth. Comment: This species is considered as a typical transition point between Strepto- myces ruber and Nocardia rubra (Krassilni- kov, op. cit., 1941, 78). Source: One strain was obtained as a mu- tant of Nocardia rubra. Another strain was changed, after 8 months of cultivation, into a typical Streptomyces. Habitat: Unknown. 37. Nocardia africana Pijper and Pul- linger, 1927. (Pijper and Pullinger, Jour. Trop. Med. and Hyg., 30, 1927, 153; Strep- tomyces africanus Waksman and Henrici, in Manual, 6th ed., 1948, 959.) af.ri.ca'na. L. adj. africanus pertaining to Africa. Description taken from Erikson (Med. Res. Council Spec. Rept. Ser. 203, 1935, 18). Unicellular, branching mycelium forming small, dense, pink colonies with short, straight, sparse, white aerial mycelium. Gelatin: Irregular pink flakes. No lique- faction. Agar: A few, flat, pink, discoid colonies. Glucose agar: Minute, red, discrete, round colonies and piled up, paler pink mass with thin white aerial mycelium. Glycerol agar: After 2 weeks, small, heaped-up, colorless masses with pink tinge around the colorless colonies; margin de- pressed; after 3 weeks, abundant, piled up, pale pink growth. Ca-agar: After 1 week, small, round, colorless colonies with red centers ; margins submerged; after 2 weeks, growth bright cherry-red, confluent, with colorless margin. Dorset's egg medium: Small, colorless blister colonies, partly confluent; become wrinkled and depressed into medium; slight liquefaction. Serum agar: Irregularly round, raised, wrinkled, colorless colonies; becoming dry, pink and flaky; later piled up, brownish, friable. Inspissated serum: After one week, smooth, round, colorless colonies with sub- merged margin, in confluent patches pink and pitted into medium; after 2 weeks, me- dium broken up, slight liquefaction; after 3 weeks, liquid dried up, colonies umbilicated, raised, dry and friable. Broth: Small pink colonies embedded in coherent flocculent mass. Synthetic sucrose solution: Small pink granules in sediment after 1 week; colonies of medium size, coherent, after 3 weeks. Potato agar: Bright red growth, small round colonies with colorless submerged margins and piled up patches with stiff', sparse, white aerial mycelium. Litmus milk: Bright red surface growth; liquid unchanged after one month; liquid
