milk coagulation, proteolysis and pigmenta- tion of aerial mycelium. Source: Isolated from soil. Habitat: Soil. 4. Streptoniyces anulatus (Beijerinck, 1912, emend. Krassilnikov, 1941) Waksman, 1953. (Streptothrix annulatus (sic) Beijerinck, Folia Microbiologica, 1, 1912, 4; Actinomyces annulatus (sic) Krassilnikov, Guide to the Actinomycetes, Izd. Akad. Nauk, U.S.S.R., Moskau, 1941, 40; Waksman, in Waksman and Lechevalier, Actinomycetes and Their Antibiotics, Baltimore, 1953, 40.) a.nu.la'tus. L. adj. anulatus furnished with a ring. Aerial mycelium: Whitish or light gray. Sphorophores produce spirals with 3 to 7 turns (sinistrorse) ; spores spherical, 0.7 micron in diameter. Gelatin: Slow liquefaction. Agar: White aerial mycelium, concentric rings less marked than those on synthetic agar. Synthetic agar: Colorless, flat growth, penetrating deep into agar. White, velvety aerial mycelium growing in the form of concentric rings. Milk: Coagulated, slowly peptonized. Sucrose is inverted. Starch is hj^drolyzed. Good growth on cellulose. Odor: Strong, earthy. Antagonistic properties: Highly antago- nistic against mycobacteria and Gram- positive bacteria; some strains are active against fungi. Source: Isolated from soil. Habitat: Soil. 5. Streptoniyces rochei Waksman, 1953. {Streptoniyces rochei Berger, Jampolsky and Goldberg (nomen nudum), Arch, of Bio- chem., 22, 1949, 477; Waksman, in Waksman and Lechevalier, Actinomycetes and Their Antibiotics, Baltimore, 1953, 40.) ro'che.i. M.L. gen. noun rochei of Roche. Description prepared by Julius Berger for use in Waksman and Lechevalier, Actino- mycetes and Their Antibiotics, Baltimore, 1953. Vegetative growth: Vegetative mycelium characteristically fine, 0.8 to 1.5 microns in diameter, with short branches on synthetic (calcium malate) and non-synthetic (pep- tone-tomato paste) agars. Aerial mycelium: Sporogenous hyphae 1.5 microns in diameter, often spirally twisted; spirals usually short and loose with rarely more than 2 to 3 coils. Spores ellip- soidal, sometimes spherical, 0.8 to 1.5 by 1.3 to 2.8 microns. Gelatin: Cream-colored surface ring, covered with white aerial mycelium. Rapid liquefaction. Faint yellow soluble pigment. Agar: Cream-colored growth. White aerial mycelium. No soluble pigment. Synthetic agar: Thin, colorless growth, covered with sandy lavender to dark gray aerial mycelium. Reverse light gray, later becoming grayish yellow. No soluble pig- ment. Ca-malate-glycerol agar: Abundant growth, raised in center. Gray aerial myce- lium, buff around the edges, having a fuzzy appearance. Medium is cleared directly under the growth. Starch agar: Brownish growth. Mouse- gray aerial mycelium. Reverse shows slight purple pigmentation in four days. Glucose agar: Smooth, yellowish growth, covered with white to gray aerial mycelium. Yellowish soluble pigment. Ca-citrate-glycerol broth: Growth at surface forming a thin mat of partially sporulated, discrete, grayish white colonies. From the mat, soft, round, fuzzy, gray colo- nies drop into the medium. Milk: Ring at the surface cream-colored to brownish. Coagulated; rapidly pepto- nized. Potato: Abundant, lichenoid, cream-col- ored growth. Abundant, cottony, white to gray aerial mycelium. Color of plug becomes reddish tan. Strong diastatic action. Antagonistic properties: On certain com- plex nitrogenous media such as those con- taining soybean flour or distillers' dried solubles, the organism produces a wide range of antimicrobial activity. Part of this is attributable to a specific antibiotic known as borrelidin. Comment": Morphologically the culture resembles some species in the Streptomyces albus group, such as Streptomyces alhido-
