Milk: Coagulated and rapidly peptonized. Sucrose rapidly inverted. Starch is rapidly hydrolyzed. Poor growth on cellulose. Nitrites actively produced from nitrates. Antagonistic properties: None; some strains are weakly active. Source: Isolated from soil. Habitat: Soil. 13. Streptomyces californicus (Waks- man and Curtis, 1916) Waksman and Henrici, 1948. (Actinomyces californicus Waksman and Curtis, Soil Sci., /, 1916, 122; Waksman and Henrici, in Manual, 6th ed., 1948, 936.) ca.li.for'ni.cus. M.L. adj. californicus pertaining to California; named for Cali- fornia, U.S.A. Aerial mycelium: Filaments with long, narrow, open spirals. Spherical to ellipsoidal conidia. Gelatin stab: Gray, moist, abundant sur- face growth. Liquefaction in 30 days. No soluble pigment. Agar: Thin, restricted, yellowish to cream-colored growth. Sj'nthetic agar: Spreading, vinaceous- colored growth. Aerial mj-celium powder}', thin, light neutral gray. No soluble pigment. Starch agar: Growth spreading, pink center with colorless to gray margin. Glucose agar: Restricted, much folded, cream-colored growth with sulfur-yellow tinge. Glucose broth: Solid, cream-colored mass on surface, with pink tinge. Litmus milk: Faint, brownish surface growth; coagulated; peptonized in 40 days. Potato: Glossy, yellow to red growth, turning red-brown. Starch is hydrolyzed. Nitrites produced from nitrates. Aerobic. Optimum temperature, 37° C. Antagonistic properties: Limited. Some strains produce viomycin. Source: Isolated once from California sandy loam. Habitat: Soil. 14. Streptomyces virgatus (Krassilni- kov, 1'941) Waksman, 1953. {Actinomyces virgatus Krassilnikov, Guide to the Actino- mycetes, Izd. Akad. Nauk, U.S.S.R., Mos- kau, 1941, 32; Waksman, in Waksman and Lechevalier, Actinomycetes and Their Anti- biotics, Baltimore, 1953, 45.) vir.ga'tus. L. adj. virgatus of twigs, rod- shaped. Vegetative growth: Yellow-green to cit- ron-yellow or pure yellow colonies; pale green on some media. Pigment insoluble in substrate. Some strains produce brown sub- stance in protein media. Aerial mycelium: Weakly developed, white or pale yellow. Sporophores produced in form of tufts. Oidiospores cylindrical, elongated; in some strains round-ellipsoidal. Gelatin: Rapid liquefaction. Milk : Rapidly coagulated and peptonized. Starch is rapidly hydrolyzed. No growth on cellulose. Nitrites actively produced from nitrates. Antagonistic properties: None. Source: Isolated from soil. Habitat: Soil. 15. Streptomyces flaveolus (Waksman, 1923) Waksman and Henrici, 1948. (Actino- myces 168, Waksman, Soil Sci., 8, 1919, 134; Acfinotnyces flaveolus Waksman, in Manual, 1st ed., 1923, 368; Waksman and Henrici, in Manual, 6th ed., 1948, 936.) fla.ve'o.lus. L. adj. flavus golden yellow; M.L. dim. adj. flaveohis somewhat yellow. Aerial mycelium: Numerous closed and open spirals on all media. Conidia ellip- soidal. Gelatin stab: Liquefied; abundant, yel- lowish, spreading pellicle. Agar: White, glistening, wrinkled growth. Synthetic agar: Growth light sulfur-yel- low turning to cadmium-yellow, penetrating deep into medium. Aerial mycelium white to ash-gray patches. Starch agar: White, spreading growth. Glucose agar: Restricted growth, surface folded, raised. Glucose broth: Thin, yellow pellicle. Litmus milk: Sulfur-yellow ring; coagu- lated; peptonized, with faintly alkaline reaction. Potato: Abundant, wrinkled, cream- colored growth. Starch is hydrolyzed.
