growth, partly covered with white mycelium on surface of medium; soluble yellow-ochre pigment. Milk: Rapid growth becoming covered with whitish aerial mycelium; never fully covering the surface; no coagulation; pep- tonization begins slowly and is completed in 13 days, liquid becoming colored yellow- ish orange. Coagulated serum: Cream-colored growth of surface becoming covered with white aerial mj-celium; rapid liquefaction of serum. Starch medium: Cream-colored growth rapidly colored with yellow aerial myce- lium; after 20 days growth becomes much folded; greenish on reverse side; slightly amber-colored in medium. Antagonistic properties: Positive. Comments: This species is closely related to Streptomyces albus. Develops poorly on synthetic media without asparagine. Source: Isolated from dust. Habitat: Unknown. 23. Streptomyces lieskei (Duche, 1934) Waksman and Henrici, 1948. {Actinomyces lieskei Duch6, Encyclop^die Mycologique, Paris, 6, 1934, 289; Waksman and Henrici, in Manual, 6th ed., 1948, 950.) lies'ke.i. M.L. gen. noun lieskei of Lieske; named for Prof. Rudolf Lieske of Leipzig. Gelatin: Cream-colored growth becoming covered with white aerial mycelium; no soluble pigment. Rapid liquefaction. Agar: Cream-colored growth becoming covered with white aerial mycelium; yel- lowish soluble pigment. Synthetic agar: Cream-colored growth with delayed white aerial mycelium grow- ing from the edge toward the center; my- celium later j^ellowish. Reverse of growth yellowish to green. Dirty yellow to yellow- green soluble pigment. Synthetic solution: Long branching fila- ments 0.7 micron in diameter. Yellowish white aerial mycelium does not readily produce spores; flakes drop to the bottom of the tube. Peptone solution: Cream-colored colo- nies on surface with flakes in the liquid dropping to the bottom of the tube. Liquid becomes yellowish in color. Tyrosine medium: Rapid growth on sur- face with whitish yellow aerial mycelium; yellowish to orange-yellow soluble pigment. Milk: Cream-colored growth; colorless on reverse side; no aerial mycelium. Peptoniza- tion without coagulation. After 20 days the whole milk becomes a clear yellowish liquid. Coagulated serum: Clear-colored growth. Rapid liquefaction. Relationship to other species: Culture related to Streptomyces alboflavus and Strep- tomyces albidoflavus . Source: Culture secured from the collec- tion of Prof. R. Lieske. Habitat: Unknown. 24. Streptomyces flavovirens (Waks- man, 1923) Waksman and Henrici, 1948. (Actinomyces 128, Waksman, Soil Sci., 8, 1919, 117; Actinomyces flavovirens Waksman, in Manual, 1st ed., 1923, 352; Waksman and Henrici, in Manual, 6th ed., 1948, 940.) fla.vo.vi'rens. L. adj. flavus yellow; L. p. adj. virens being green; M.L. adj. flavo- virens being yellow-green. Aerial mycelium: Large masses of minute tufts; the hyphae coarse, straight, short, relatively unbranched, beaded; open spirals may be produced in certain substances. Conidia spherical, ellipsoidal to rod-shaped, 0.75 to 1.0 by 1.0 to 1.5 microns. Gelatin stab: Yellowish green surface pellicle, consisting of a mass of small colo- nies, on the liquefied medium. Agar: Yellowish growth; the reverse dark in center with yellowish zone and outer white zone. Synthetic agar: Growth spreading deep into the substratum, yellowish with green- ish tinge. Aerial mycelium gray, powdery. Starch agar: Greenish yellow, spreading growth, developing deep into the medium. Glucose agar: Restricted growth, de- veloping only to a very small extent into the medium, yellow, turning black, edge entire. Glucose broth: Thick, sulfur-yellow pel- licle or ring. Litmus milk: Cream-colored to brownish ring; coagulated; peptonized, becoming faintly alkaline. Potato: Sulfur-yellow, wrinkled growth. Starch is hydrolyzed.
