Page:Bergey's manual of determinative bacteriology.djvu/790

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ORDER V. ACTINOMYCETALES

Agar: Cream-colored growth. Thin aerial mycelium. Sj^nthetic agar: Thin, gray, spreading growth. Aerial mycelium white, becoming drab gray. Starch agar: Thin, colorless, spreading growth. Aerial mycelium gray. Glucose agar: Yellowish, spreading growth. No aerial mycelium. Glucose broth: Gray ring with grayish colonies in bottom of tube. Litmus milk: Brownish ring; coagulated; peptonized in 25 to 30 days, becoming faintly alkaline. Potato: Abundant, wrinkled, cream- colored growth with greenish tinge. Starch is hydrolyzed. Brown to dark brown soluble pigment formed. Nitrites produced from nitrates. Aerobic. Optimum temperature, 37° C. Antagonistic properties: Limited. Source: Isolated from soil. Habitat: Soil. 35. Streptomyces canescens Hickey et al., 1952. {Streptomijces canescus (sic) Hickey, Corum, Hidy, Cohen, Nager and Kropp, Antibiotics and Chemotherapy, 2, 1952, 472.) ca.nes'cens. L. part. adj. canescens be- coming white, or hoary. Aerial mycelium: At first white, becom- ing, on sporulation, gray-white to gray. Conidiophores straight or curved, not form- ing any spirals, richly septate. Spores globose, 1.0 to 1.3 by 1.3 to 2.6 microns. Gelatin: Rapid liquefaction. Sabouraud's agar: Growth first white, dull-shiny, spreading, translucent with tan reverse. After 7 days' incubation, growth beaded, slightly wrinkled at base of the slant, grayish white; reverse tan to amber. Amber pigment diffused throughout me- dium. After 14 days aerial mycelium faintly greenish. Bennett's agar: Circular colonies, effuse to conve.x, edge filamentous; powdery; vary- ing from gray-white to gray. Reverse brown. No soluble pigment. Egg medium: Tan, very wrinkled surface growth. No sporulation observed after 10 days' incubation; small amount of white sporulation observed in 14 days. Soluble brown pigment. After 21 days, odor of hy- drogen sulfide detected. Liquefaction after 28 days. Ca-malate agar: Gray to rose-gray my- celium with yellow to tan reverse. Diges- tion of calcium malate slight at edge of colony. No soluble pigment. Glucose, arabinose, trehalose, xylose, su- crose, maltose, galactose, dextrin, soluble starch, mannitol, glycerol and salicin are used as sole sources of carbon. No growth observed with sorbose, melezitose, dulcitol, rhamnose, sorbitol, melibiose, phenol, raffinose or lactose. Milk: At 36° C, becomes alkaline (pH 8.4). Soft, rennet curd, formed after 48 hours, is completely peptonized in 12 days. Potato: Light gray, spreading, wrinkled growth. Deep brown pigment diffused throughout. Starch is actively hydrolyzed. Nitrites not produced from nitrates. C. Produces a contaminated Optimum temperature, I Antagonistic properties cosin. Source : Isolated from fungus plate. Habitat: Unknown. 36. Streptomyces finiicarius (Duch^, 1934) Waksman and Henrici, 1948. {Actino- myces fimicarius Duche, Encyclop^die My- cologique, Paris, 6, 1934, 346; Waksman and Henrici, in Manual, 6th ed., 1948, 940.) fi.mi.ca'ri.us. L. noun fim us dung, ma- nure; L. adj. cams dear, loving; M.L. adj. fimicarius dung-loving. Gelatin: Punctiform colonies with whitish aerial mycelium; reddish soluble pigment. Liquefaction. Agar: Cream-colored growth with white aerial mycelium; reverse side, yellowish. Asparagine agar: Cream-colored growth with whitish aerial mycelium; reverse side cream-colored to slight ochre. Synthetic agar: Yellowish masses of growth with j^ellowish white aerial my- celium; reverse side orange-colored; faint yellowish soluble pigment. Asparagine solution: Vegetative filaments 0.5 to 0.6 micron long; branching aerial