colored to straw-colored. No soluble pig- ment. Glucose agar: Cream-colored to brownish growth. Aerial mycelium abundant, cream- colored. Faint soluble brownish pigment. Broth: Colorless surface film. Aerial my- celium heavy, cream-colored. No soluble pigment. Milk: Colorless to brownish surface ring. No aerial mycelium. Not coagulated; pep- tonized. Potato: Folded, brownish growth. Aerial mj^celium cream-colored. Faint, soluble pigment. Sucrose is inverted. Starch is actively hydrol.yzed. Good growth on cellulose. Paraffin and fats support good growth. Nitrites produced from nitrates. Odor: Strong, characteristic of soil. Antagonistic properties: Some strains give positive effects, others are negative. Source : Isolated from sputum in a case of chronic bronchitis. Habitat: Unknown. 74. Strep tomyces roseoflavus Arai, 1951. (Jour. Antibiotics (Japanese), 4, 1951, 215; see Biol. Abst., 21, 1953, No. 1867.) ro.se. o.fia'vus. L. fem.n. rosa a rose; L. adj . jlavus yellow; M.L. adj . roseoflavus rose- yellow. Vegetative growth: Large, flat colonies; vegetative mycelium limited to medium. Aerial mycelium: White to rose-colored, producing numerous spirals. Spores ellip- soidal to oblong, 0.8 to 1.0 by 1.0 to 1.8 microns. Gelatin: Strong liquefaction. Orange- brown colonies at bottom of liquefied zone. No soluble pigment. Agar: Much-folded, white-gray to golden j^ellow growth. White to rose-colored aerial mycelium, limited to center of colonies. Sjmthetic agar: Colorless to j-ellowish growth, penetrating deep into medium. Powdery, white to .yellow-rose aerial my- celium. Starch agar: Golden yellow growth with whitish aerial mycelium. Glucose agar: Colorless to yellowish white growth with rose-colored aerial mj^celium. Milk: Cream-colored surface ring. Rap- idly coagulated and peptonized, the medium becoming strongly alkaline. Potato: Yellow mass without aerial my- celium. No soluble pigment. Fair growth on cellulose paper; cellulose is decomposed. Nitrites produced from nitrates. Antagonistic properties : Produces a basic antibiotic, flavomycin, similar to neomycin. Comment: Culture similar to that of Streptomyces microflavus. Source: Isolated from garden soil from Chiba and Hiroshima. Habitat: Soil. 75. Streptomyces putrificus (Nicolai- eva, 1915) Waksman, 1953. [Actinomyces puirificus Nicolaieva, Arch. Biol. Nauk, 18, 1915, 240; Streptomyces putrificans (sic) Waksman, in Waksman and Lechevalier, Actinomj^cetes and Their Antibiotics, Bal- timore, 1953, 81.) pu.tri'fi.cus. L. V. putrefacio to make rotten; M.L. adj. putrificus making rotten. Vegetative growth: Colorless. Aerial mycelium: White. Agar: Grayish growth covered with white aerial mycelium. No soluble pigment. LoefHer's serum: Yellow growth. No aerial mycelium. Serum liquefied and colored j'ellowish brown. Glucose broth: Yellow soluble pigment. Milk: Heavy pellicle, covered with white aerial mycelium. Gradually peptonized without previous coagulation. Potato: Folded, sulfur-j-ellow growth. Aerial mycelium chalk-white. No soluble pigment. Proteins energetically decomposed with the production of bad-smelling products, hydrogen sulfide and ammonia. Odor: Strong, putrefactive. Relationships to other species : Similar to Actinomyces albus Krainsky but differs from it in that it putrefies proteins with the production of gases. Source : Isolated from surface water near St. Petersburg. Habitat: Unknown. 76. Streptomyces citreus (Krainsky, 1914) Waksman and Henrici, 1948. (Actino- myces citreus Krainsky, Cent. f. Bakt., II
