Page:Bergey's manual of determinative bacteriology.djvu/821

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FAMILY III. STREPTOMYCETACEAE
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growth; fine white aerial mycelium; slow liquefaction of serum. Source: Culture obtained from Mr. Malengon, an inspector in Morocco. Habitat: Unknown. 104. Streptoniyces aiiliniycoticus Waksman, nom. nov. {Streptoniyces sp., Leben, Stessel and Keitt, M^-cologia, 44, 1952, 159.) an.ti.mj'.co'ti.cus. Gr. pref. anti against; Gr. noun myces fungus; M.L. adj. anti- mycoticus against -fungus. Vegetative growth: On most media early growth at first white and later gray; the color change appears to be associated with the development of dense masses of spores. Aerial mycelium: Spirals situated typi- cally in dense groups along the main por- tions of aerial hyphae, especially toward the center of the colony. In early formation of sporing hyphae, spirals tend to be open; they become closed and compact, however, prior to the formation of spores. In late stages these fruiting structures tend to appear ball-like rather than spiral. Spores ellipsoidal, hyaline and 0.6 to 1.3 by 0.7 to 2.0 microns; diameter of spore spirals, 4 to 8 microns. Gelatin: Vegetative mycelium translu- cent, cream-colored; sparse, white aerial mycelium. Liquefaction slight at 15 days, moderate at 30 days. No soluble pigment. Agar: Shiny, cream-colored vegetative mycelium; moderate, pebbly, white aerial mycelium. No soluble pigment. Synthetic agar: Abundant, pebbly, light neutral gray aerial mycelium. No soluble pigment. Glucose asparagine agar: Same as on synthetic agar. Glucose peptone agar: Same as on syn- thetic agar. Ca-malate-glycerol agar: Abundant, peb- bly, light neutral gray aerial mj'celium. Faint green soluble pigment. Potato glucose agar: Abundant, pebbly, light neutral gray aerial mycelium. Faint brown soluble pigment. Yeast extract agar: Same as on synthetic agar. Starch: Abundant, pebblj', white to neu- tral gray aerial mycelium. No soluble pig- ment. Glucose broth: Surface colonies coalesc- ing, white, powdery. Liquid clear, not pigmented. Small amount of flaky sediment present. Milk: Cream-colored ring. Coagulated; peptonized in 15 to 30 days, becoming slightly alkaline. Yellowish orange serum, slightly turbid. Potato: Finely wrinkled, cream-colored growth with sparse, white aerial mycelium. Plug darkened slightly. Diastatic action weak to moderate. Nitrites weakly produced from nitrates. Antagonistic properties: Produces an antifungal agent, helixin. Source: Isolate A 158 from compost soil. Habitat: Soil. 105. Streptomyces fradiae (Waksman and Curtis, 1916) Waksman and Henrici, 1948. {Actinomyces fradii (sic) Waksman and Curtis, Soil Sci., 1, 1916, 125; Waksman and Henrici, in Manual, 6th ed., 1948, 954.) fra'di.ae. M.L. gen. noun fradiae of Fradia; named for a person, Fradia. Aerial mycelium: Straight, branching filaments and hyphae. No spirals. Conidia rod-shaped or ellipsoidal, 0.5 by 0.7 to 1.25 microns. Gelatin stab: Cream-colored to brownish, dense growth on liquid medium. Agar: Yellowish growth, becoming or- ange-yellow, restricted. No soluble pig- ment . Synthetic agar: Smooth, spreading, color- less growth. Aerial mycelium thick, cottony mass covering surface, sea-shell pink. Starch agar: Spreading, colorless growth. Glucose agar: Growth restricted, glossy, buff-colored, lichenoid margin. Glucose broth: Dense, narrow, orange- colored ring; abundant, flaky, colorless sediment. Litmus milk: Faint, cream-colored ring; coagulated; peptonized, becoming alkaline. Potato: Restricted, orange-colored growth. The pigment formed is not soluble. Starch is hydrolyzed. Nitrites not produced from nitrates. Aerobic.