Page:Bergey's manual of determinative bacteriology.djvu/830

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ORDER V. ACTINOMYCETALES

with abundant, white to green to olive-buff aerial mj'celium. Soluble, brownish to black pigment. Nitrites produced from nitrates. Starch is hydrolyzed. Grows well at 37.5° C. Comment: According to Krassilnikov, Streptomyces flavus, Streptomyces margina- tus, Streptomyces praefecundus , Streptomyces tenuis and Streptomyces loidensis represent merely strains of this species. Source: Isolated from a small, unruptured potato scab. Habitat: Found in potatoes so far as known. 125. Streptomyces praefecundus (Mil- lard and Burr, 1926) Waksman, 1953. (Ac- tinomyces praefecundus Millard and Burr, Ann. Appl. Biol., 13, 1926, 601; Waksman, in Waksman and Lechevalier, Actinomy- cetes and Their Antibiotics, Baltimore, 1953, 108.) prae.fe.cun'dus. L. adj. praefecundus very fruitful. Aerial mycelium: Spores ellipsoidal, 0.8 by 0.85 micron. Gelatin: Good surface growth covered with aerial mycelium. Light pink to dark golden brown soluble pigment. Rapid lique- faction. Synthetic sucrose agar: Good, cream- colored growth covered with abundant, cottony, olive-buff aerial mycelium. Soluble cream-colored pigment. Synthetic glucose agar: Pale olive-gray growth covered with abundant, smooth, olive-buff aerial mycelium. Soluble, light golden to buff pigment. Potato agar: Lichenoid, gray growth. Aerial mycelium smooth, white to yellowish. Soluble golden brown pigment. Glycerol synthetic solution: Heavy sur- face growth covered with white to olive-buff aerial mycelium. Soluble pigment none or very slightly green. Glucose broth: Masses throughout me- dium flaky, whitish. Aerial mycelium abun- dant, pale olive-buff. Soluble, light golden brown pigment. Milk: Good surface growth covered with scant, white aerial mycelium. Coagulation followed by peptonization. Potato: Good, wrinkled growth covered with abundant, white to yellowish to olive - buff aerial mycelium. Soluble, gray to brown pigment. Starch is hydrolyzed. Tyrosinase reaction: Negative. Nitrites produced from nitrates. Grows well at 37.5° C. Source: Isolated from a large, ruptured potato scab. Habitat: Found in potatoes and soil so far as known. 126. Streptomyces viridogenes Waks- man, 1953. (Actinomyces viridis Millard and Burr, Ann. Appl. Biol., IS, 1926, 601; not Actinomyces viridis Sanfelice, Cent. f. Bakt., I Abt., Grig., 36, 1904, 355; Waksman, in Waksman and Lechevalier, Actinomycetes and Their Antibiotics, Baltimore, 1953, 109.) vi.ri.do'ge.nes. L. adj. viridis green; Gr. v.suffix -genes producing; M.L. adj. viri- dogenes green-producing. Aerial mycelium: Long, straight fila- ments. Spores spherical, 0.9 micron. Gelatin: Grayish growth with scant, white to gray, aerial mycelium. Soluble light golden brown pigment. Rapid liquefaction. Agar: Lichenoid, cream-colored growth. No aerial mycelium. No soluble pigment. Sj^nthetic sucrose agar: Abundant growth covered with olive-grayish aerial mycelium. Soluble greenish yellow to blackish green pigment . Synthetic glucose agar: Smooth, raised, olive-buff growth. Abundant aerial myce- lium, light gray to deep mouse-gray. Solu- ble yellowish to greenish yellow pigment. Glucose agar: Gray to black growth. Aerial mj'celium gray. Soluble dark brown pigment. Starch agar: Gray to brown growth. Aerial mycelium thin, white. Broth: Thin surface growth and flaky, cream-colored growth in bottom. No solu- ble pigment. Glycerol synthetic solution: Flaky, white to vinaceous growth in medium and on surface. Aerial mycelium gray. Glucose broth: Growth on surface and at base good, flakj'. Aerial mycelium gray to mouse-gray. Soluble vinaceous to cinnamon- colored pigment.