Serum agar: Wrinkled, glistening, cream- colored, membranous growth. Inspissated serum: Colorless smeary growth, reverse becoming transparent, starting to liquefy at base; completely liquefied and brown in 12 days. Broth: Suspended and sedimented color- less flocculi, some small round colonies. Synthetic sucrose solution: Abundant white colonies in coherent mass near bot- tom of tube; large shell -shaped masses. Synthetic glycerol solution: At first, a few round white colonies in suspension; later, large branched feathery mass at bottom. Milk: Coagulated; later peptonized. Litmus milk: Medium deep blue, be- coming hydrolyzed to clear purple. Potato plug: Colorless moist membranous growth with scant white aerial mycelium at top of plug. Starch is hydrolyzed. Tyrosine agar: Reaction negative. Source: Isolated from human spleen in a case of splenic anemia. Habitat: Unknown. 146. Streptomyces kimberi (Erikson, 1935) Waksman and Henrici, 1948. (Actino- myces kimberi Erikson, Med. Res. Council Spec. Rept. Ser. 203, 1935, 36; Waksman and Henrici, in Manual, 6th ed., 1948, 964.) kim'be.ri. M.L. gen. noun kimberi of Kimber; presumably named for the surgeon who first secured this organism. Vegetative growth: Mycelium of long, straight, profusely branching filaments forming circumscribed colonies on all media. Aerial mycelium: Abundant production of short, straight and branched aerial my- celium; small round conidia. Non-acid-fast. Gelatin: Liquefaction. Smooth shining colonies becoming powdery white with aerial mycelium, floating on liquefied me- dium. No pigmentation. Agar: Smooth round moist cream-colored colonies, 1 mm in diameter; after 17 days, white powdery aerial mycelium. Glucose agar: Discrete cream-colored colonies becoming confluent, white aerial mycelium. Glycerol agar: Moist cream-colored colo- nies becoming confluent, white aerial my- celium. Potato agar: Extensive growth covered by white powdery aerial mycelium; large colorless exuded droplets. Wort agar: Heavy brownish lichenoid colony; after 30 days, a white aerial my- celium. Ca-agar: Dull cream-colored scaly growth, covered by chalky white aerial mycelium. Coon's agar: Extensive growth, white aerial mycelium in annular arrangement. Synthetic agar: Small colonies covered with white aerial mycelium. Blood agar: Many large colonies, cream- colored, tough, smooth, glistening, with margin depressed; no hemolysis. Serum agar: Moist, cream-colored honey- combed skin, scant white aerial mycelium. Dorset's egg medium: Closely adherent scale-like colonies, centrally elevated, with white aerial mycelium. Inspissated serum: Rapid spreading growth, discrete round colonies at margin, completely covered with white aerial my- celium, colorless transpired drops; slight softening at base. Broth: Small round colonies in sediment in 2 days; supernatant colonies with white aerial mycelium and large hollow flakes in sediment in 15 days; occasional reddish brown coloration. Synthetic sucrose solution: Round white colonies at bottom; later small stellate colonies in suspension and a few superna- tant with white aerial mycelium. Synthetic glycerol solution : Round white colonies at bottom; later coherent mulberry- like mass composed of fluffy round portions ; after 15 days, irregular wispy flocculi and large coherent mass. Milk: Coagulation; no peptonization; initial pinkish brown ring descends until medium is dark brown throughout (2 months). Litmus milk: Blue coloration, hydrolyzed to clear purple in 2 months. Starch not hydrolyzed. Tyrosine agar: Reaction negative. Source: Isolated from a blood culture of a woman with acholuric jaundice.
