broth cultures do not discolor suspensions of horse erythrocj'tes. Coagulated blood serum: No liquefaction. Hj'drogen sulfide production is slight. Carbohydrates not attacked. Methylene blue is rapidly reduced. Aerobic; poor growth under anaerobic con- ditions. Serologically different from Mycoplasma mycoides var. mycoides and M. mycoides var. capri. Pathogenicity : Goats are more susceptible than sheep to experimental infection by sub- cutaneous inoculation; the inflammatory lesions are localized in the udders of females and, in 10 to 20 per cent of the cases, in the joints. Non-pathogenic for mice and other laboratory animals. Source: Isolated from cases of contagious agalactia of sheep and goats. Habitat: The etiological agent of contagi- ous agalactia of sheep and goats. 4. Mycoplasma spiimans Edward, 1955. (a strains of dogs, Edward and Fitzgerald, Jour. Gen. Microbiol., 5, 1951, 566; Edward, in Freundt, Internat. Bull, of Bact. Nomen. and Taxon., 5, 1955, 73; see Edward, In- ternat. Bull, of Bact. Nomen. and Taxon., 5, 1955, 90.) spu'mans. L. part. adj. spumans foaming. Unstable and sparsely branched mycelioid structure with very short, almost bacillary filaments which usually measure 2 to 5 microns in length (Freundt, unpublished ob- servation). Gram-negative. Horse-serum agar: Upon initial isolation and in early subcultures, the colonies are coarsely reticulated, and the centers con- tain large globules; colonial characteristics are lost on repeated subculture. Neither film nor spots are produced. Horse-blood agar: Trace of hemolj^sis. Rabbit-serum agar: Poor growth. Semi-solid media: Growth throughout the medium is neither definitely smooth nor definitely granular. Carbohydrates not attacked. Methylene blue is slowly reduced. Aerobic, facultatively anaerobic. Serologically distinct from the other mem- bers of this genus by agglutinin adsorption and complement fixation tests. Pathogenicity: Unknown. Source: Isolated from the vagina and semen of dogs. 5. Mycoplasma canis Edward, 1955. (/3 strains of dogs, Edward and Fitzgerald, Jour. Gen. Microbiol., 5, 1951, 566; Edward, in Freundt, Internat. Bull, of Bact. Nomen. and Taxon., 5, 1955, 73; see Edward, Inter- nat. Bull, of Bact. Nomen. and Taxon., 5, 1955, 90.) ca'nis. L. noun canis the dog. Unstable and sparsely branched mycelioid structure with very short, almost bacillary filaments which usually measure 2 to 5 microns in length (Freundt, unpublished ob- servation). Gram-negative. Horse-serum agar: Relativelj^ large colo- nies with poorly developed central spots in early subcultures. Neither film nor spots are produced. Horse-blood agar: Pronounced alpha he- molysis. Rabbit-serum agar: Good growth. Semi -solid media: Growth throughout the medium is usually neither definitely smooth nor definitely granular; a few strains pro- duce a typical smooth growth. Carbohydrates not attacked. Methylene blue is reduced rather rapidly. Aerobic, facultatively anaerobic. Serologically distinct from the other mem- bers of this genus. Pathogenicity: Unknown. Source: Isolated from the throats and genital tracts of dogs. Habitat: Commonly found in the genital tract and throat of dogs. 6. Mycoplasma maculosiim Edward, 1955. (7 strains of dogs, Edward and Fitz- gerald, Jour. Gen. Microbiol., 5, 1951, 566; Edward, in Freundt, Internat. Bull, of Bact. Nomen. and Taxon., 5, 1955,73; see Edward, Internat. Bull, of Bact. Nomen. and Taxon., 5, 1955, 90.) ma.cu.lo'sum. L. adj. niaculosus spotted. Unstable and sparsely branched mycelioid structure with very short, almost bacillary filaments which usually measure 2 to 5 mi- crons in length (Freundt, unpublished ob- servation). Gram-negative. Horse-serum agar: A film and spots are produced.
