V. The etiological agent of mouse pneumonitis (Gonnert). 5. Miyagawanella bronchopneuniojiiae. VI. The etiological agent of feline pneumonitis (Baker). 6. Miyagawanella felis. VII. The etiological agent of Louisiana pneumonitis. 7. Miyagawanella louisianae. VIII. The etiological agent called the Illinois virus; the cause of one type of viral pneu- monia. 8. Miyagawanella illinii. IX. The etiological agent of paralysis in opossums; opossum A virus (Roca-Garcia). 9. Miyagawanella opossumi. X. The etiological agent of ovine abortion (Stamp, McEwen, Watt and Nisbet). 10. Miyagawanella ovis. XI. An agent from the intestinal tract of calves (York and Baker). 11. Miyagawanella bovis. XII. The etiological agent of sporadic bovine encephalomyelitis (McNutt). 12. Miyagawanella pecoris. 1. Miyagawanella lymphogranuloma- tosis Brumpt, 1938. (Brumpt, Ann. de Parasitol., 16, 1938, 155; Ehrlichia lymph- ogranulomatosis Moshkovskiy, Uspekhi Souremennoi Biologii (Russian) (Advances in Modern Biology), 19, 1945; Rickettsiafor- mis lymphogranulomatis Zhdanov and Kor- enblit. Jour. Microbiol., Epidemiol, and Immunobiol. (Russian), No. 9, 1950, 43 (type species (by subsequent designation, Philip, Canad. Jour. Microbiol., 3, 1956, 265) of genus Rickettsiaformis Zhdanov and Kor- enblit,op.ai.,1950, 43; Chlamydozoon lymph- ophilus Ryzhkov, Voprosy Meditsinskoi Virusologii (Problems of Med. Virology), Akad. Med. Nauk, S.S.S.R., Moskau, 3, 1950, 17.) lym.pho.gran.u.lo.ma.to'sis. M.L. fem.n. lymphogranulomatosis the disease lymphogranulomatosis; M.L. gen. noun lymphogranulomatosis of lymphogranuloma- tosis. Coccoid cells 200 to 350 millimicrons in diameter are the elementary bodies. Initial bodies up to 1 micron and plaques up to 10 microns in diameter are also found. All larger forms are encapsulated with a sub- stance derived either from the agent or from the cytoplasm of parasitized cells. The ele- mentary body is the basic unit. Occur in pairs or clusters. Non-motile. Stain with aniline dyes. Stain purple with Giemsa's and red or blue, depending on the metabolic state, with Macchiavello's stain. The ma- trix of the plaque does not give the reaction for glycogen. Gram-negative. Filterability: Passes through Chamber- land L2 and L3 , Berkefeld V and N and sometimes through Seitz EK filters. Cultivation: Growth occurs in tissue cul- tures of mammalian cells, in mammalian cells on agar and in the chorio-allantoic membrane, and particularly in the yolk sac, of the chicken embryo but not in the allan- toic sac. Optimum temperature, 37° C. in tissue cultures and 35° C. in the chicken embryo. Immunology: Possesses one or more anti- gens in common with or closely resembling one or more of those present in the chlamy- diae and in other miyagawanellae. Antisera against any of the species in these two gen- era react with antigens from Miyagawanella lymphogranulomatosis or the other miyaga- wanellae thus far tested. One common anti- gen has been isolated as a soluble fraction distinct from the bodies of the agent. Sharply distinguished from the other miya- gawanellae by antitoxic neutralization of the toxic factor or by neutralization of in- fections in mice with chicken antisera. Evi- dence exists that these two serological reac- tions are with distinct specific antigens. Immunity in man or other animals is proba- bly poor in the absence of continuing appar- ent or inapparent infection. Toxic factor: High concentrations of this agent in infected yolk sac or in yolk injected intravenously or intraperitoneally are rap- idly fatal to mice. Produces characteristic lesions on the skin of normal guinea pigs.
