OXFORD 
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GigaScience, 7, 2018, 1–13 doi: 10.1093/gigascience/giy095 Advance Access Publication Date: 7 August 2018 Data Note |
DATA NOTE
Draft genome assembly of the invasive cane toad, Rhinella marina
Richard J. Edwards1, Daniel Enosi Tuipulotu1†, Timothy G. Amos1†, Denis O'Meally2, Mark F. Richardson3,4, Tonia L Russell5, Marcelo Vallinoto6,7, Miguel Carneiro6, Nuno Ferrand6,8,9, Marc R. Wilkins1,5, Fernando Sequeira6, Lee A. Rollins3,10, Edward C. Holmes11, Richard Shine12 and Peter A. White
1,*
1School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia, 2Sydney School of Veterinary Science, Faculty of Science, University of Sydney, Camperdown, NSW, 2052, Australia, 3School of Life and Environmental Sciences, Centre for Integrative Ecology, Deakin University, Geelong, VIC, 3216, Australia, 4Bioinformatics Core Research Group, Deakin University, Geelong, VIC, 3216, Australia, 5Ramaciotti Centre for Genomics, University of New South Wales, Sydney, NSW, 2052, Australia, 6CIBIO/InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, Portugal, 7Laboratório de Evolução, Instituto de Estudos Costeiros (IECOS), Universidade Federal do Pará, Bragança, Pará, Brazil, 8Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Porto, Portugal, 9Department of Zoology, Faculty of Sciences, University of Johannesburg, Auckland Park, South Africa, 10Evolution and Ecology Research Centre, School of Biological Earth and Environmental Sciences, University of New South Wales, Sydney, NSW, 2052, Australia, 11Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, University of Sydney, Sydney, NSW, 2006, Australia and 12School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Camperdown, NSW, 2006, Australia
*Corresponding address. Peter A. White, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia. Tel: +61-293853780; E-mail: p.white@unsw.edu.au 
http://orcid.org/0000-0002-6046-9631
†These authors contributed equally to this work.
Abstract
Background: The cane toad (Rhinella marina formerly Bufo marinus) is a species native to Central and South America that has spread across many regions of the globe. Cane toads are known for their rapid adaptation and deleterious impacts on native fauna in invaded regions. However, despite an iconic status, there are major gaps in our understanding of cane toad genetics. The availability of a genome would help to close these gaps and accelerate cane toad research. Findings: We report a draft genome assembly for R. marina, the first of its kind for the Bufonidae family. We used a combination of long-read Pacific Biosciences RS II and short-read Illumina HiSeq X sequencing to generate 359.5 Gb of raw sequence data.
Received: 22 March 2018; Revised: 4 June 2018; Accepted: 22 July 2018
© The Author(s) 2018. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
