many cells still contained spheres, here and there one could be seen filled with purple fluid, without a vestige of aggregated protoplasm; the whole having been redissolved. A leaf with aggregated masses, caused by its having been waved for 2 m. in water at the temperature of 125 Fahr., was left in cold water, and after 11 hrs. the protoplasm showed traces of incipient redissolution. When again examined three days after its immersion in the warm water, there was a conspicuous difference, though the protoplasm was still somewhat aggregated. Another leaf, with the contents of all the cells strongly aggregated from the action of a weak solution of phosphate of ammonia, was left for between three and four days in a mixture (known to be innocuous) of one drachm of alcohol to eight drachms of water, and when re-examined every trace of aggregation had disappeared, the cells being now filled with homogeneous fluid.
We have seen that leaves immersed for some hours in dense solutions of sugar, gum, and starch have the contents of their cells greatly aggregated, and are rendered more or less flaccid, with the tentacles irregularly contorted. These leaves, after being left for four days in distilled water, became less flaccid, with their tentacles partially re-expanded, and the aggregated masses of protoplasm were partially redissolved. A leaf with its tentacles closely clasped over a fly, and with the contents of the cells strongly aggregated, was placed in a little sheny wine; after 2 hrs. several of the tentacles had re-expanded, and the others could by a mere touch be pushed back into their properly expanded positions, and now all traces of aggregation had disappeared, the cells being filled with perfectly homogeneous pink fluid. The redissolution in these cases may, I presume, be attributed to endosmose.
On the Proximate Causes of the Process of Aggregation.
As most of the stimulants which cause the inflection ai the tentacles likewise induce aggregation in the contents of their cells, this latter process might be thought to be the direct result of inflection ; but this is not the case. If leaves are placed in rather strong solutions of carbonate of ammonia, for instance of three or four, and even sometimes of only two grains to the ounce of water (i. e. one part to 109, or 146, or 218, of water), the tentacles are paralyzed, and do not become inflected, yet they soon exhibit strongly marked aggregation. Moreover, the short central tentacles of a leaf which has been immersed in a weak solution of any salt of ammonia, or in any nitrogenous organic fluid, do not become in the least inflected; nevertheless, they exhibit all the phenomena of aggregation. On the other hand, several acids cause strongly pronounced inflection, but no aggregation.
