ANILINE, o-TOLUIDINE, AND NITROBENZENE
Aniline C6H7N o-Toluidine C7H9N
Nitrobenzene C6H5NO2 METHOD: 2017, Issue 1
MW: 93.13 MW: 107.2 MW: 123.1
CAS: 62-53-3 CAS: 95-53-4 CAS: 98-95-3
RTECS: BW6650000 RTECS: XU2975000 RTECS: DA6475000
EVALUATION: PARTIAL
OSHA: Table 1 NIOSH: Table 1 ACGIH: Table 1
2017
Issue 1: 15 January 1998
PROPERTIES: Table 1
SYNONYMS: Aniline: aminobenzene, benzenamine, phenylamine o-Toluidine: o-aminotoluene, 2-aminotoluene, 1-methyl-2-aminobenzene, o-methylaniline, 2-methylaniline Nitrobenzene: nitrobenzol, oil of mirbane SAMPLING
MEASUREMENT
SAMPLER: FILTER + SOLID SORBENT (glass fiber filter, sulfuric acid treated + silica gel sorbent tube, 520/260 mg) Alternate samplers: gauze wipes, 4 x 4 in.; dermal badge sampler (1 g silica gel in cotton pouch)
TECHNIQUE:
GAS CHROMATOGRAPHY, FID
ANALYTES:
aniline, o-toluidine, nitrobenzene
DESORPTION:
2 mL ethanol
FLOW RATE: 0.2 L/min [1]
INJECTION VOLUME:
1 µL
VOL-MIN: -MAX:
5L 50 L
TEMPERATURE-INJECTION: 250 °C -DETECTOR: 300 °C -COLUMN: 35 °C to 150 °C (8 °C/min)
SHIPMENT: routine SAMPLE STABILITY: aniline and o-toluidine 7 days @ 5 °C [2]; nitrobenzene 60 days [1] BLANKS:
CARRIER GAS:
helium, 2.4 mL/min
COLUMN:
capillary column, 30 m, 0.32-mm ID,1-µm film, (Rtx-5, or equivalent) [1]
CALIBRATION:
solutions of analytes in ethanol
RANGE:
Table 2 [1]
2 to 10 field blanks per set ACCURACY
RANGE STUDIED:
Table 2
BIAS:
not determined
ESTIMATED LOD: Table 2 [1] OVERALL PRECISION ( ): not determined ACCURACY:
PRECISION ( ):
Table 2 [1]
not determined
APPLICABILITY: Under the GC parameters stated in the method, aniline, o-toluidine, and nitrobenzene can be identified based upon retention time and quantified. Analyte recovery was optimized under conditions of moderate relative humidity (< 53%), low sampling rates (0.2 L/min), and sample volumes < 50 L [1]. Sulfuric acid-treated glass fiber filters were used to collect aniline and o-toluidine, while nitrobenzene was collected on the silica gel sorbent [1]. Nitrobenzene collection on silica gel tubes was significantly reduced under conditions of high relative humidity [1]. Dermal badge samplers (cotton pouch with 1 g silica gel) and gauze wipes were developed to estimate exposures via splashes, spills, and passive collection of aerosol vapors. INTERFERENCES: No specific interferences were identified. However, any compound with a similar retention time may interfere. OTHER METHODS: This method revises and combines methods 2002 (aniline and o-toluidine) [3], and 2005 (nitrobenzene) [4].
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition
