ANISIDINE
CH3OC6H4NH2
MW: 123.16
METHOD: 2514, Issue 2
CAS: (o-) 90-04-0 (p-) 104-94-9
2514
RTECS:
EVALUATION: FULL
OSHA : 0.5 mg/m 3 (skin) NIOSH: 0.5 mg/m 3 (skin); o-isomer suspect carcinogen ACGIH: 0.1 ppm (skin) (0.5 mg/m 3) (1 ppm = 5.03 mg/m 3 @ NTP)
PROPERTIES:
(o-) BZ5410000 (p-) BZ5450000
Issue 1: 15 May 1985 Issue 2: 15 August 1994
o-isomer: liquid; d 1.092 g/mL @ 15 °C; BP 225 °C; MP 5 °C; VP 0.1 mm Hg @ 27 °C p-isomer: solid; MP 57 °C; BP 246 °C
SYNONYMS: o-isomer: 2-aminoanisole; 2-methoxybenzenamine; o-methoxyaniline p-isomer: 4-aminoanisole; 4-methoxybenzenamine; p-methoxyaniline
SAMPLING SAMPLER:
SOLID SORBENT TUBE (XAD-2, 150 mg/75 mg)
MEASUREMENT TECHNIQUE:
HPLC, UV DETECTION
ANALYTE:
o-anisidine and p-anisidine
DESORPTION:
5 mL methanol; stand 15 min
INJECTION VOLUME:
10 µL
FLOW RATE: 0.5 to 1.0 L/min VOL-MIN: -MAX:
24 L 320 L
SHIPMENT:
routine
SAMPLE STABILITY:
at least 1 week @ 25 C
BLANKS:
2 to 10 field blanks per set
ACCURACY RANGE STUDIED:
0.13 to 0.58 mg/m 3 [1] (225-L samples)
BIAS:
0.12%
MOBILE PHASE:
35% acetonitrile/65% water @ 1.2 mL/min; ambient temperature
COLUMN:
50 cm x 2-mm ID stainless steel packed with µ-Bondapak C 18 or equivalent
DETECTOR:
UV absorption @ 254 nm
CALIBRATION:
analytes dissolved in methanol
RANGE:
12 to 360 µg per sample (each isomer) [2]
ESTIMATED LOD: 0.35 µg per sample [2]
ˆ rT): 0.068 [1] OVERALL PRECISION (S ACCURACY:
± 13.3%
PRECISION (Sr):
0.029 @ 30 to 240 µg per sample [1]
APPLICABILITY: The working range is 0.06 to 0.8 mg/m 3 (0.012 to 0.16 ppm) for a 200-L air sample.
INTERFERENCES: None identified.
OTHER METHODS: This revises Method S163 [2]. This method replaces P&CAM 168 [3] because XAD-2 has a much greater capacity than silica gel for o-anisidine at high humidity [1].
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
