ISOPHORONE C9 H14 O
MW: 138.21
METHOD: 2556, Issue 1
OSHA : NIOSH: ACGIH:
CAS: 78-59-1
2556 RTECS: GW7700000
EVALUATION: PARTIAL
25 ppm 4 ppm; Group III Pesticide C 5 ppm (animal carcinogen)
PROPERTIES:
Issue 1: 15 March 2003
liquid; d 0.923 g/mL @ 25 °C; BP 213 °C; VP 26 kPa (0.2 mm Hg 260 ppm) @ 20 °C
SYNONYMS: 3,5,5-trimethyl-2-cyclohexen-1-one
SAMPLING SAMPLER:
SOLID SORBENT TUBE (XAD-4, 80 mg/40 mg)
FLOW RATE:
0.01 to 1 L/min
VOL-MIN: -MAX:
2 L @ 25 ppm 25 L
SHIPMENT:
Ship cold.
MEASUREMENT TECHNIQUE:
GAS CHROMATOGRAPHY, FID
ANALYTE:
Isophorone
EXTRACTION:
1 mL diethyl ether; stand 30 min and rotate 1.5 hours
INJECTION VOLUME: 1 µL
SAMPLE STABILITY:
At least 14 days @ 5 °C
BLANKS:
2 to 10 field blanks per set
ACCURACY
RANGE STUDIED:
Not Determined.
ACCURACY:
Not Determined.
BIAS:
Not Determined.
OVERALL PRECISION (S r T ):
Not Determined.
TEMPERATURE -INJECTOR: -DETECTOR: -COLUMN:
240 °C 300 °C 50 °C (0.5 min) to 225°C (10 °C/min)
CARRIER GAS:
He, 3.0 mL/min
COLUMN:
Capillary, fused silica 30-m x 0.32mm ID; 1 µm film crossbond carbowax- PEG for acidic compounds
CALIBRATION:
isophorone in diethyl ether
RANGE:
6 to 831 µg per sample [1]
ESTIMATED LOD:
1.0 µg per sample [1]
PRECISION (þ r ):
0.01 [1]
APPLICABILITY: The working range for this method is 0.042 to 5.88 ppm (0.24 to 33.2 mg/m3 ) for a 25-L air sample. High humidity may greatly decrease breakthrough volume.
INTERFERENCES: None identified.
OTHER METHODS: This method was developed for air sampling at lower concentrations of isophorone. NMAM Method 2508, for isophorone, is based on the 2nd edition method S367 [2,3]. This older method uses a petroleum-based charcoal sorbent tube and packed-column gas chromatography.
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition
