ETHYLENIMINE
C 2H 5N
MW: 43.07
METHOD: 3514, Issue 2
CAS: 151-56-4
3514
RTECS: KX5075000
EVALUATION: FULL
OSHA : carcinogen NIOSH: carcinogen ACGIH: 0.5 ppm (skin) (1 ppm = 1.76 mg/m 3)
PROPERTIES:
Issue 1: 2 May 1979 Issue 2: 15 August 1994
liquid; mp, -74.0 °C; bp, 56-57 °C; d 0.8321 g/mL @ 24 °C; VP 21 kPa (160 mm Hg) @ 20 °C
SYNONYMS: aziridine, azacyclopropane
SAMPLING SAMPLER:
BUBBLER (Folin's reagent)
MEASUREMENT TECHNIQUE:
HPLC, UV DETECTION
ANALYTE:
4-(1-aziridinyl)-1,2-naphthoquinone
EXTRACTION:
4 mL of CHCl 3, 15 sec. (twice)
FLOW RATE: 0.2 L/min VOL-MIN: -MAX:
1 L @ 0.5 ppm 48 L
FINAL VOLUME:
SHIPMENT:
ship at 5 °C in dark
INJECTION VOLUME: 10 µL
SAMPLE STABILITY:
14 days @ 5 °C in dark
BLANKS:
2 to 10 field blanks per set
10 mL
MOBILE PHASE:
59.5% hexane:40% chloroform (with 1% ethanol):0.5% 2-propanol
FLOW RATE:
1.3 mL/min
COLUMN:
Lichrosorb DIOL 25 cm x 4.6-mm)
DETECTOR:
UV @ 254 nm
CALIBRATION:
4-(1-aziridinyl)-1,2-naphthoquinone CHCl 3
RANGE:
1 to 795 µg per sample
ACCURACY RANGE STUDIED:
0.16 to 21 mg/m 3 (2.6- to 39-L samples)
BIAS:
- 2%
ˆ ): 0.069 OVERALL PRECISION (S rT ACCURACY:
± 15.5%
in
ESTIMATED LOD: 0.3 µg per sample PRECISION (Sr):
0.024
APPLICABILITY: The working range is 0.014 to 11 ppm (0.025 to 20 mg/m 3) for a 40-L air sample. A variation of this method has been used to measure ethylenimine in air at 0.02 to 0.10 mg/m 3 at a manufacturing plant [1,2]. The modifications of the method included use of a µBondapak-CN column in place of the Lichrosorb DIOL column and 50:48:2 hexane:dichloromethane:2 propanol for the mobile phase.
INTERFERENCES: Propylenimine and 2-bromoethylamine interfere.
OTHER METHODS: This method replaces Method P&CAM 300 [3] and is a variation of the method of Evans et al. [4]. The product of ethylenimine and Folin's reagent can be measured spectrophotometrically [5,6].
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
